ArticleJournal of bacteriology2015
Role of the Streptococcus mutans CRISPR-Cas systems in immunity and cell physiology.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 71 citations in OpenAlex.
- Defense Systems and Prophage Detection in Streptococcus mutans Strains.Molecular oral microbiology · 2026Article
- MerR-type regulator ZccR links zinc homeostasis to biofilm formation and interspecies competition inFrontiers in cellular and infection microbiology · 2026Article
- Transcription regulator TrtA modulates oxygen stress response inApplied and environmental microbiology · 2025Article
- The stress of carrying CRISPR-Cas.Virulence · 2025Article
- Acquired CRISPR spacers and rhamnose-glucose polysaccharide defects confer resistance toMicrobiology (Reading, England) · 2025Article
- Article
- Acquired CRISPR spacers and rhamnose-glucose polysaccharide defects confer resistance tobioRxiv : the preprint server for biology · 2025Article
- Role of CRISPR-Cas systems in periodontal disease pathogenesis and potential for periodontal therapy: A review.Molecular oral microbiology · 2025Review
- Human saliva modifies growth, biofilm architecture, and competitive behaviors of oral streptococci.mSphere · 2024Article
- Function of the RNA-targeting class 2 type VI CRISPR Cas system ofFrontiers in microbiology · 2024Article
- CcpA and CodY Regulate CRISPR-Cas System ofMicrobiology spectrum · 2023Article
- Mechanisms regulating the CRISPR-Cas systems.Frontiers in microbiology · 2023Review
- Prospective Advances in Genome Editing Investigation.Advances in experimental medicine and biology · 2023Article
- CRISPR-Cas systems: role in cellular processes beyond adaptive immunity.Folia microbiologica · 2022Review
- Distribution, Diversity and Roles of CRISPR-Cas Systems in Human and Animal Pathogenic Streptococci.Frontiers in microbiology · 2022Review
- Investigating CRISPR spacer targets and their impact on genomic diversification ofFrontiers in genetics · 2022Article
- Article
- Characterization of FtsH Essentiality inFrontiers in genetics · 2021Article
- [Transcriptomic Analysis ofSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2021Article
- New Insights onFrontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
CRISPR-Cas systems provide adaptive microbial immunity against invading viruses and plasmids. The cariogenic bacterium Streptococcus mutans UA159 has two CRISPR-Cas systems: CRISPR1 (type II-A) and CRISPR2 (type I-C) with several spacers from both CRISPR cassettes matching sequences of phage M102 or genomic sequences of other S. mutans. The deletion of the cas genes of CRISPR1 (ΔC1S), CRISPR2 (ΔC2E), or both CRISPR1+2 (ΔC1SC2E) or the removal of spacers 2 and 3 (ΔCR1SP13E) in S. mutans UA159 did not affect phage sensitivity when challenged with virulent phage M102. Using plasmid transformation experiments, we demonstrated that the CRISPR1-Cas system inhibits transformation of S. mutans by the plasmids matching the spacers 2 and 3. Functional analysis of the cas deletion mutants revealed that in addition to a role in plasmid targeting, both CRISPR systems also contribute to the regulation of bacterial physiology in S. mutans. Compared to wild-type cells, the ΔC1S strain displayed diminished growth under cell membrane and oxidative stress, enhanced growth under low pH, and had reduced survival under heat shock and DNA-damaging conditions, whereas the ΔC2E strain exhibited increased sensitivity to heat shock. Transcriptional analysis revealed that the two-component signal transduction system VicR/K differentially modulates expression of cas genes within CRISPR-Cas systems, suggesting that VicR/K might coordinate the expression of two CRISPR-Cas systems. Collectively, we provide in vivo evidence that the type II-A CRISPR-Cas system of S. mutans may be targeted to manipulate its stress response and to influence the host to control the uptake and dissemination of antibiotic resistance genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.