ArticleAntimicrobial agents and chemotherapy2015
Lactate as a novel quantitative measure of viability in Schistosoma mansoni drug sensitivity assays.
Article in Antimicrobial agents and chemotherapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Extracellular Vesicles From Schistosoma mansoni Adult Worms Stimulate IL-10 Release by B Cells.Parasite immunology · 2025Article
- Characterization of Schistosome Sox Genes and Identification of a Flatworm Class of Sox Regulators.Pathogens (Basel, Switzerland) · 2023Article
- Phenotypic Profiling of Macrocyclic Lactones on ParasiticAntimicrobial agents and chemotherapy · 2023Article
- Optimizing the xWORM assay for monitoring hookworm larvae motility.Frontiers in parasitology · 2023Article
- A self-purifying microfluidic system for identifying drugs acting against adult schistosomes.Royal Society open science · 2022Article
- Genetic analysis of praziquantel response in schistosome parasites implicates a transient receptor potential channel.Science translational medicine · 2021Article
- Natural-Product-Based Solutions for Tropical Infectious Diseases.Clinical microbiology reviews · 2021Review
- High-content approaches to anthelmintic drug screening.Trends in parasitology · 2021Review
- Evidence for in vitro and in vivo activity of the antimalarial pyronaridine against Schistosoma.PLoS neglected tropical diseases · 2021Article
- Identification of hit compounds with anti-schistosomal activity on in vitro generated juvenile worms in cell-free medium.PLoS neglected tropical diseases · 2021Article
- In vitro screening as an anthelmintic discovery pipeline for Calicophoron daubneyi: nutritive media and rumen environment-based approaches.Parasitology research · 2021Article
- Nitric oxide debilitates the neuropathogenic schistosome Trichobilharzia regenti in mice, partly by inhibiting its vital peptidases.Parasites & vectors · 2020Article
- Inhibition of the NAD salvage pathway in schistosomes impairs metabolism, reproduction, and parasite survival.PLoS pathogens · 2020Article
- The antioxidants resveratrol and N-acetylcysteine enhance anthelmintic activity of praziquantel and artesunate against Schistosoma mansoni.Parasites & vectors · 2019Article
- Molluscicidal activities of curcumin-nisin polylactic acid nanoparticle on Biomphalaria pfeifferi.PLoS neglected tropical diseases · 2017Article
- Susceptibility Testing of Medically Important Parasites.Clinical microbiology reviews · 2017Review
- A high-throughput colorimetric assay for detection of Schistosoma mansoni viability based on the tetrazolium salt XTT.Parasites & vectors · 2017Article
- Modern approaches to accelerate discovery of new antischistosomal drugs.Expert opinion on drug discovery · 2016Review
- Mechanically produced schistosomula as a higher-throughput tools for phenotypic pre-screening in drug sensitivity assays: current research and future trends.Biomarker research · 2016Review
- Fluorescence/luminescence-based markers for the assessment of Schistosoma mansoni schistosomula drug assays.Parasites & vectors · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole-organism compound sensitivity assays are a valuable strategy in infectious diseases to identify active molecules. In schistosomiasis drug discovery, larval-stage Schistosoma allows the use of a certain degree of automation in the screening of compounds. Unfortunately, the throughput is limited, as drug activity is determined by manual assessment of Schistosoma viability by microscopy. To develop a simple and quantifiable surrogate marker for viability, we targeted glucose metabolism, which is central to Schistosoma survival. Lactate is the end product of glycolysis in human Schistosoma stages and can be detected in the supernatant. We assessed lactate as a surrogate marker for viability in Schistosoma drug screening assays. We thoroughly investigated parameters of lactate measurement and performed drug sensitivity assays by applying schistosomula and adult worms to establish a proof of concept. Lactate levels clearly reflected the viability of schistosomula and correlated with schistosomulum numbers. Compounds with reported potencies were tested, and activities were determined by lactate assay and by microscopy. We conclude that lactate is a sensitive and simple surrogate marker to be measured to determine Schistosoma viability in compound screening assays. Low numbers of schistosomula and the commercial availability of lactate assay reagents make the assay particularly attractive to throughput approaches. Furthermore, standardization of procedures and quantitative evaluation of compound activities facilitate interassay comparisons of potencies and, thus, concerted drug discovery approaches.
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