ArticleIn vitro cellular & developmental biology. Animal2015
Role of calpastatin in the regulation of mRNA expression of calpain, caspase, and heat shock protein systems in bovine muscle satellite cells.
Article in In vitro cellular & developmental biology. Animal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed, 23 citations in OpenAlex.
- Determining the Presence of a Polymorphism in the Calpastatin (Life (Basel, Switzerland) · 2025Article
- Aging mechanism for improving the tenderness and taste characteristics of meat.Journal of animal science and technology · 2023Review
- Association of single nucleotide polymorphisms in the CAPN, CAST, LEP, GH, and IGF-1 genes with growth parameters and ultrasound characteristics of the Longissimus dorsi muscle in Colombian hair sheep.Tropical animal health and production · 2022Article
- Whole Genome Sequence Data Provides Novel Insights Into the Genetic Architecture of Meat Quality Traits in Beef.Frontiers in genetics · 2020Article
- MicroRNA expression analysis identifies a subset of downregulated miRNAs in ALS motor neuron progenitors.Scientific reports · 2018Article
- Association of μ-Calpain and Calpastatin Polymorphisms with Meat Tenderness in a Brahman-Angus Population.Frontiers in genetics · 2018Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calpastatin participates in apoptotic cell death and cell signaling, but its role in skeletal myoblast development and molecular involvements in cell growth still remains unknown. The current study aimed to investigate the role of calpastatin on the expression patterns of calpains, caspases, and heat shock proteins (HSPs). In addition, the cell viability during myoblast growth under calpastatin silence condition was also investigated. Three small interference RNA sequences (siRNAs) were used to silence calpastatin gene and ligated into pSilencer plasmid vector to construct short hairpin RNA (shRNA) expression. The all three siRNAs significantly silence the calpastatin gene. Moreover, suppression of calpastatin significantly reduced the viability of myoblasts during growth phase when compared to control cells. Additionally, knockdown of calpastatin significantly increased the mRNA expression of μ-calpain, caspase-3, caspase-7, and caspase-9, as well as HSP-27, -70, and -90. The present study results suggested that the suppression of calpastatin resulted in the increased expression of μ-calpain, caspases, and HSPs which in turn regulate the apoptotic cell death. The present study throws light on the central role of calpastatin in the control of calpain activity, cell proliferation, cell survival, and apoptotic pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.