Evidence map›Paper›PMID 25487434›Full record

ArticleScientific reports2014

Separable bilayer microfiltration device for viable label-free enrichment of circulating tumour cells.

Ming-Da Zhou, Sijie Hao, Anthony J Williams, Ramdane A Harouaka, Brett Schrand, Siddarth Rawal, Zheng Ao, Randall Brenneman, Eli Gilboa, Bo Lu and 6 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
4.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 107 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 1 country.

Ming-Da ZhouMicro &Nano Integrated Biosystem (MINIBio) Laboratory, Department of Biomedical Engineering and Materials Research Institute, Pennsylvania State University, University Park, PA 16802, U.S.A.
Sijie HaoMicro &Nano Integrated Biosystem (MINIBio) Laboratory, Department of Biomedical Engineering and Materials Research Institute, Pennsylvania State University, University Park, PA 16802, U.S.A.
Anthony J Williams1] Department of Pathology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A. [2] Sheila and David Fuente Graduate Program in Cancer Biology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A. [3] Dr John T Macdonald Foundation Biomedical Nanotechnology Institute, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A.
Ramdane A HarouakaMicro &Nano Integrated Biosystem (MINIBio) Laboratory, Department of Biomedical Engineering and Materials Research Institute, Pennsylvania State University, University Park, PA 16802, U.S.A.
Brett Schrand1] Sheila and David Fuente Graduate Program in Cancer Biology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A. [2] Department of Microbiology and Immunology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A.
Siddarth Rawal1] Department of Pathology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A. [2] Dr John T Macdonald Foundation Biomedical Nanotechnology Institute, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A.
Zheng Ao1] Department of Pathology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A. [2] Sheila and David Fuente Graduate Program in Cancer Biology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A. [3] Dr John T Macdonald Foundation Biomedical Nanotechnology Institute, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A.
Randall Brenneman1] Sheila and David Fuente Graduate Program in Cancer Biology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A. [2] Department of Microbiology and Immunology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A.
Eli GilboaDepartment of Microbiology and Immunology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A.
Bo LuCaltech Micromachining Laboratory, California Institute of Technology, MC 136-93, Pasadena, CA 91125, U.S.A.
Shuwen WangDepartment of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99210, U.S.A.
Jiyue ZhuDepartment of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA 99210, U.S.A.
Ram Datar1] Department of Pathology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A. [2] Dr John T Macdonald Foundation Biomedical Nanotechnology Institute, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A.
Richard Cote1] Department of Pathology, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A. [2] Dr John T Macdonald Foundation Biomedical Nanotechnology Institute, University of Miami - Miller School of Medicine, Miami, FL 33136, U.S.A.
Yu-Chong TaiCaltech Micromachining Laboratory, California Institute of Technology, MC 136-93, Pasadena, CA 91125, U.S.A.
Si-Yang ZhengMicro &Nano Integrated Biosystem (MINIBio) Laboratory, Department of Biomedical Engineering and Materials Research Institute, Pennsylvania State University, University Park, PA 16802, U.S.A.
University of Miami · USPennsylvania State University · USCalifornia Institute of Technology · USWashington State University Spokane · USDr. John T. Macdonald Foundation · US

Funding

Integration of Flexible Micro Spring Array and High Throughput Microfluidics forDP2CA174508 · NCI · PENNSYLVANIA STATE UNIVERSITY, THE · PI ZHENG, SIYANG · 2012 to 2012
$2.2M
NCI NIH HHS DP2 CA174508NCI NIH HHS DP2CA174508
6 · The paper itself

Abstract

The analysis of circulating tumour cells (CTCs) in cancer patients could provide important information for therapeutic management. Enrichment of viable CTCs could permit performance of functional analyses on CTCs to broaden understanding of metastatic disease. However, this has not been widely accomplished. Addressing this challenge, we present a separable bilayer (SB) microfilter for viable size-based CTC capture. Unlike other single-layer CTC microfilters, the precise gap between the two layers and the architecture of pore alignment result in drastic reduction in mechanical stress on CTCs, capturing them viably. Using multiple cancer cell lines spiked in healthy donor blood, the SB microfilter demonstrated high capture efficiency (78-83%), high retention of cell viability (71-74%), high tumour cell enrichment against leukocytes (1.7-2 × 10(3)), and widespread ability to establish cultures post-capture (100% of cell lines tested). In a metastatic mouse model, SB microfilters successfully enriched viable mouse CTCs from 0.4-0.6 mL whole mouse blood samples and established in vitro cultures for further genetic and functional analysis. Our preliminary studies reflect the efficacy of the SB microfilter device to efficiently and reliably enrich viable CTCs in animal model studies, constituting an exciting technology for new insights in cancer research.

Indexed as

Micropore FiltersAnimalsCell Line, TumorCell SeparationCell SurvivalDisease Models, AnimalHumansMiceNeoplastic Cells, CirculatingReproducibility of Results

Identifiers

PMID25487434
PMCPMC4260227
OpenAlexW2066791831

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.