ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2015
Partial uracil-DNA-glycosylase treatment for screening of ancient DNA.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 272 papers.
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Who cites it
272 citing papers in PubMed, 577 citations in OpenAlex.
- What modern genomes overlook: Ancient DNA rewrites the phylogeography of European atlantic salmon (iScience · 2026Article
- A minimal hybridization capture approach for the parallel enrichment and cost-effective detection of ancient human pathogens.iScience · 2026Article
- A refined phylochronology of the second plague pandemic in Western Eurasia.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Population genomics, demography, and circum-Baltic connectivity of Early Medieval southwestern Finland.Genome biology · 2026Article
- The Oral Microbiome of King Richard III of England.American journal of biological anthropology · 2026Article
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- Jomon genomics reveal cold adaptation in Upper Paleolithic hunter-gatherers of eastern Eurasia.Science advances · 2026Article
- De novo assembly and authentication of ancient DNA metagenomes with nf-core/mag.PLoS computational biology · 2026Article
- A damage-aware NGS workflow for conservative species identification from ultra-degraded DNA.Analytical and bioanalytical chemistry · 2026Article
- Ancient genomics study reveals low HLA diversity in eastern hunter-gatherers.Genome biology · 2026Article
- Ancient genomes from Ladakh reveal 2800-year-old admixture between Tibetans and South Asians.Science advances · 2026Article
- Genetic identification of Árpád Dynasty members from the ossuary of the Royal Basilica at Székesfehérvár.iScience · 2026Article
- Ancient mitogenomes reveal the genetic relationship among extinct and extant water buffaloes.iScience · 2026Article
- DNA signatures preserved in the official 1978 sample collection of the Shroud of Turin.Scientific reports · 2026Article
- The genetic landscape of northeastern Iberian communities from the early to late Iron Age.iScience · 2026Article
- Mesopotamian ancient DNA reveals Iron Age integration of heterogeneous Bronze Age genetic ancestries following resettlement.Genome biology · 2026Article
- The genetic legacy of the 17th-century colonial capital of St. Mary's City.Current biology : CB · 2026Article
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- Bioarchaeological analysis illustrates the life of a 16th-century Sámi individual from Kitka, Kuusamo, northern Finland.BMC genomics · 2026Article
212 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The challenge of sequencing ancient DNA has led to the development of specialized laboratory protocols that have focused on reducing contamination and maximizing the number of molecules that are extracted from ancient remains. Despite the fact that success in ancient DNA studies is typically obtained by screening many samples to identify a promising subset, ancient DNA protocols have not, in general, focused on reducing the time required to screen samples. We present an adaptation of a popular ancient library preparation method that makes screening more efficient. First, the DNA extract is treated using a protocol that causes characteristic ancient DNA damage to be restricted to the terminal nucleotides, while nearly eliminating it in the interior of the DNA molecules, allowing a single library to be used both to test for ancient DNA authenticity and to carry out population genetic analysis. Second, the DNA molecules are ligated to a unique pair of barcodes, which eliminates undetected cross-contamination from this step onwards. Third, the barcoded library molecules include incomplete adapters of short length that can increase the specificity of hybridization-based genomic target enrichment. The adapters are completed just before sequencing, so the same DNA library can be used in multiple experiments, and the sequences distinguished. We demonstrate this protocol on 60 ancient human samples.
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