ArticleeLife2014
Ambiguities in helical reconstruction.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Helicon: Helical parameter determination and 3D reconstruction from one image.Journal of structural biology · 2025Article
- Cryo-EM for atomic characterization of supramolecular gels.Faraday discussions · 2025Article
- Structural insights intomBio · 2025Review
- GSK3β phosphorylated tau filaments are probably not like those from AD brains.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cryo-EM reconstruction of helical polymers: Beyond the simple cases.Quarterly reviews of biophysics · 2024Review
- Near-atomic-resolution structure of J-aggregated helical light-harvesting nanotubes.Nature chemistry · 2024Article
- Helical reconstruction, again.Current opinion in structural biology · 2024Review
- Helical reconstruction of VP39 reveals principles for baculovirus nucleocapsid assembly.Nature communications · 2024Article
- Enzymatic control of intermolecular interactions for generating synthetic nanoarchitectures in cellular environment.Science and technology of advanced materials · 2024Review
- Helical reconstruction of VP39 reveals principles for baculovirus nucleocapsid assembly.bioRxiv : the preprint server for biology · 2023Article
- Helical Indexing in Real Space.Scientific reports · 2022Article
- On bias, variance, overfitting, gold standard and consensus in single-particle analysis by cryo-electron microscopy.Acta crystallographica. Section D, Structural biology · 2022Article
- Enzymatic Noncovalent Synthesis for Targeting Subcellular Organelles.ChemPlusChem · 2022Review
- Python-based Helix Indexer: A graphical user interface program for finding symmetry of helical assembly through Fourier-Bessel indexing of electron microscopic data.Protein science : a publication of the Protein Society · 2022Article
- Article
- Cryo-EM is a powerful tool, but helical applications can have pitfalls.Soft matter · 2021Article
- Cryoelectron-Microscopic Structure of the pKpQIL Conjugative Pili from Carbapenem-Resistant Klebsiella pneumoniae.Structure (London, England : 1993) · 2020Article
- Artificial Intracellular Filaments.Cell reports. Physical science · 2020Article
- Atomic structure of theProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Amyloid structure determination in RELION-3.1.Acta crystallographica. Section D, Structural biology · 2020Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
Helical polymers are found throughout biology and account for a substantial fraction of the protein in a cell. These filaments are very attractive for three-dimensional reconstruction from electron micrographs due to the fact that projections of these filaments show many different views of identical subunits in identical environments. However, ambiguities exist in defining the symmetry of a helical filament when one has limited resolution, and mistakes can be made. Until one reaches a near-atomic level of resolution, there are not necessarily reality-checks that can distinguish between correct and incorrect solutions. A recent paper in eLife (Xu et al., 2014) almost certainly imposed an incorrect helical symmetry and this can be seen using filament images posted by Xu et al. A comparison between the atomic model proposed and the published three-dimensional reconstruction should have suggested that an incorrect solution was found.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.