ArticleAnalytical chemistry2015
Discovery of a heparan sulfate 3-O-sulfation specific peeling reaction.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed.
- Synthetic biology for heparin biomanufacturing.Bioresources and bioprocessing · 2026Review
- Synthesis of 4-azido sialic acid for testing against Siglec-7 and in metabolic oligosaccharide engineering.RSC chemical biology · 2025Article
- Investigation of sample handling steps for accurate heparan sulphate disaccharide analysis using HPLC-MS.Electrophoresis · 2025Article
- Untangling Heparan Sulfate 3-Analytical chemistry · 2025Article
- Increased 3-Science advances · 2023Article
- Investigation of the pharmacokinetic properties of synthetic heparan sulfate oligosaccharides.Glycobiology · 2023Article
- Molecular dynamics-based descriptors of 3-O-Sulfated Heparan sulfate as contributors of protein binding specificity.Computational biology and chemistry · 2022Article
- Emerging chemical and biochemical tools for studying 3-American journal of physiology. Cell physiology · 2022Review
- Influence of saccharide modifications on heparin lyase III substrate specificities.Glycobiology · 2022Article
- Dissecting structure-function of 3-O-sulfated heparin and engineered heparan sulfates.Science advances · 2021Article
- Peracylation Coupled with Tandem Mass Spectrometry for Structural Sequencing of Sulfated Glycosaminoglycan Mixtures without Depolymerization.Journal of the American Society for Mass Spectrometry · 2020Article
- Synthesis of 3-Biochemistry · 2020Article
- (Semi)-Synthetic Fucosylated Chondroitin Sulfate Oligo- and Polysaccharides.Marine drugs · 2020Review
- ZNF263 is a transcriptional regulator of heparin and heparan sulfate biosynthesis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Heparanase as an Additional Tool for Detecting Structural Peculiarities of Heparin Oligosaccharides.Molecules (Basel, Switzerland) · 2019Article
- Oligosaccharides from depolymerized fucosylated glycosaminoglycan: Structures and minimum size for intrinsic factor Xase complex inhibition.The Journal of biological chemistry · 2018Article
- Software for Peak Finding and Elemental Composition Assignment for Glycosaminoglycan Tandem Mass Spectra.Molecular & cellular proteomics : MCP · 2018Article
- Negative Electron Transfer Dissociation Sequencing of 3-O-Sulfation-Containing Heparan Sulfate Oligosaccharides.Journal of the American Society for Mass Spectrometry · 2018Article
- Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.Analytical chemistry · 2016Article
- De Novo Sequencing of Complex Mixtures of Heparan Sulfate Oligosaccharides.Analytical chemistry · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection. However, the low natural abundance of HS 3-O-sulfation poses a serious challenge for functional studies other than the two cases mentioned above. By contrast, multiple distinct isoforms of 3-O-sulfotranserases exist in mammals (up to seven isoenzymes). Here we describe a novel peeling reaction that specifically degrades HS chains with 3-O-sulfated glucosamine at the reducing-end. When HS/heparin is enzymatically depolymerized for compositional analysis, 3-O-sulfated glucosamine at the reducing ends appears to be susceptible to degradation under mildly basic conditions. We propose a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed. Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
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Registered trials
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