ArticleCell cycle (Georgetown, Tex.)2014
Different fates of oocytes with DNA double-strand breaks in vitro and in vivo.
Article in Cell cycle (Georgetown, Tex.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- BRCA Mutations, DNA Repair Deficiency, and Ovarian Aging.Biology of reproduction · 2015Pooled it
- Rapamycin reduces DNA damage of in vitro matured oocytes by promoting Rad51-mediated homologous recombination.Reproductive biology and endocrinology : RB&E · 2025Article
- Beneficial effects of melatonin on canine oocyte nuclear maturation via reduction of oxidative stress.Reproduction (Cambridge, England) · 2025Article
- Increased DNA damage in full-grown oocytes is correlated with diminished autophagy activation.Nature communications · 2024Article
- The Future of Breast Cancer Research in the Survivorship Field.Oncology and therapy · 2023Review
- Rapamycin improves the quality and developmental competence of mice oocytes by promoting DNA damage repair during in vitro maturation.Reproductive biology and endocrinology : RB&E · 2022Article
- Review
- Proteins from the DNA Damage Response: Regulation, Dysfunction, and Anticancer Strategies.Cancers · 2021Review
- Ageing and ovarian stimulation modulate the relative levels of transcript abundance of oocyte DNA repair genes during the germinal vesicle-metaphase II transition in mice.Journal of assisted reproduction and genetics · 2021Article
- Burning Questions in the Oncofertility Counseling of Young Breast Cancer Patients.Breast cancer : basic and clinical research · 2020Review
- Inhibition of PTEN activates bovine non-growing follicles in vitro but increases DNA damage and reduces DNA repair response.Human reproduction (Oxford, England) · 2019Article
- Autophagy is required for proper meiosis of porcine oocytes maturing in vitro.Scientific reports · 2018Article
- The capacity of oocytes for DNA repair.Cellular and molecular life sciences : CMLS · 2018Review
- Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation.Scientific reports · 2017Article
- RAD51 maintains chromosome integrity and mitochondrial distribution during porcine oocyte maturation in vitro.The Journal of reproduction and development · 2017Article
- DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes.Scientific reports · 2017Article
- Protein Phosphatase 6 Protects Prophase I-Arrested Oocytes by Safeguarding Genomic Integrity.PLoS genetics · 2016Article
- The role of Rad51 in safeguarding mitochondrial activity during the meiotic cell cycle in mammalian oocytes.Scientific reports · 2016Article
- Role of the Polymerase ϵ sub-unit DPB2 in DNA replication, cell cycle regulation and DNA damage response in Arabidopsis.Nucleic acids research · 2016Article
- Multiple Small Supernumerary Marker Chromosomes Resulting from Maternal Meiosis I or II Errors.Molecular syndromology · 2016Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In female mice, despite the presence of slight DNA double-strand breaks (DSBs), fully grown oocytes are able to undergo meiosis resumption as indicated by germinal vesicle breakdown (GVBD); however, severe DNA DSBs do reduce and delay entry into M phase through activation of the DNA damage checkpoint. But little is known about the effect of severe DNA DSBs on the spindle assembly checkpoint (SAC) during oocyte maturation. We showed that nearly no first polar body (PB1) was extruded at 12 h of in vitro maturation (IVM) in severe DNA DSBs oocytes, and the limited number of oocytes with PB1 were actually at telophase. However, about 60% of the severe DNA DSBs oocytes which underwent GVBD at 2 h of IVM released a PB1 at 18 h of IVM and these oocytes did reach the second metaphase (MII) stage. Chromosome spread at MI and MII stages showed that chromosomes fragmented after GVBD in severe DNA DSBs oocytes. The delayed PB1 extrusion was due to the disrupted attachment of microtubules to kinetochores and activation of the SAC. At the same time, misaligned chromosome fragments became obvious at the first metaphase (MI) in severe DNA DSBs oocytes. These data implied that the inactivation of SAC during the metaphase-anaphase transition of first meiosis was independent of chromosome integrity. Next, we induced DNA DSBs in vivo, and found that the number of superovulated oocytes per mouse was significantly reduced; moreover, this treatment increased the percentage of apoptotic oocytes. These results suggest that DNA DSBs oocytes undergo apoptosis in vivo.
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