ArticleF1000Research2014
Characterization of M-laurdan, a versatile probe to explore order in lipid membranes.
Article in F1000Research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 21 citations in OpenAlex.
- Lipidomic profiling reveals age-dependent changes in plasma membrane lipids that affect neural stem cell aging.Science advances · 2026Article
- Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids.Current topics in membranes · 2021Review
- Phthiocerol Dimycocerosates FromFrontiers in cellular and infection microbiology · 2020Article
- Mechanical properties of plasma membrane vesicles correlate with lipid order, viscosity and cell density.Communications biology · 2019Article
- Dynamic pattern generation in cell membranes: Current insights into membrane organization.Biochimica et biophysica acta. Biomembranes · 2018Review
- Using spectral decomposition of the signals from laurdan-derived probes to evaluate the physical state of membranes in live cells.F1000Research · 2017Article
- Characterization of M-laurdan, a versatile probe to explore order in lipid membranes.F1000Research · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microdomains corresponding to localized partition of lipids between ordered and less ordered environments are the subject of intensive investigations, because of their putative participation in modulating cellular responses. One popular approach in the field consists in labelling membranes with solvatochromic fluorescent probes such as laurdan and C-laurdan. In this report, we describe a high-yield procedure for the synthesis of laurdan, C-laurdan and two new fluorophores, called MoC-laurdan and M-laurdan, as well as their extensive photophysical characterization. We find that the latter probe, M-laurdan, is particularly suited to discriminate lipid phases independently of the chemical nature of the lipids, as measured by both fluorescence Generalized Polarization (GP) and anisotropy in large unilamellar vesicles made of various lipid compositions. In addition, staining of live cells with M-laurdan shows a good stability over time without any apparent toxicity, as well as a wider distribution in the various cell compartments than the other probes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.