ArticleJundishapur journal of microbiology2014
Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP.
Article in Jundishapur journal of microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Review
- A one-step closed-tube enzyme-activated blocked probe assay based on SNP for rapid detection of Salmonella Pullorum.Poultry science · 2021Article
- Identification and Discrimination ofFrontiers in microbiology · 2018Article
- Phenotypic and genotypic characterization of locally isolatedVeterinary world · 2016Article
- Genomic-Based Restriction Enzyme Selection for Specific Detection of Piscirickettsia salmonis by 16S rDNA PCR-RFLP.Frontiers in microbiology · 2016Article
- One-Step Identification of Five Prominent Chicken Salmonella Serovars and Biotypes.Journal of clinical microbiology · 2015Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. Among Salmonella spp., Salmonella gallinarum and Salmonella. pullorum are the most common causative agents of chicken salmonellosis resulting in high mortality and morbidity.
objectivesThe aim of this study was to identify S. gallinarum and S. pullorum by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. MATERIALS AND
methodsIn this study, 13 samples of Salmonella, isolated from local poultry, were obtained from Razi Type Culture Collection (RTCC). For the PCR-RFLP method based on the fliC gene, extracted DNA was used as a template for amplifying of the fliC gene (197bp) using specific primers. PCR products were subjected to digestion using Hinp1I restriction endonuclease.
resultsFor the PCR, 197 bp fliC fragment was amplified from all 13 isolates. Ten out of 13 were S. gallinarum and the other three were S. pullorum. As part of the PCR-RFLP, two fragments were obtained (82 bp and 115 bp) for all S. gallinarum, whereas no digestion was observed in S. pullorum, and 197 bp fragment was seen.
conclusionsPCR-RFLP with fliC gene and Hinp1I endonuclease were successfully applied to differentiate the two biotypes. The results suggested that this technique could be effective in detecting S. gallinarum and S. pullorum.
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