ArticleCurrent biology : CB2015
Nuclear size scaling during Xenopus early development contributes to midblastula transition timing.
Article in Current biology : CB, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- Nuclear size and physical properties of the nucleoplasm are determined by colloid osmotic pressure at the nuclear envelope.bioRxiv : the preprint server for biology · 2026Article
- Getting nuclear size just right - emerging mechanisms regulating nuclear scaling and morphology.Journal of cell science · 2026Review
- Reconstitution of genome remodeling usingFrontiers in cell and developmental biology · 2026Review
- Regulation of zygotic genome activation by the nucleocytoplasmic ratio.Frontiers in cell and developmental biology · 2026Review
- Visualizing developmental dynamics of nuclear morphology and transport machinery in Drosophila.Developmental biology · 2025Article
- Conserved nucleocytoplasmic density homeostasis drives cellular organization across eukaryotes.Nature communications · 2025Article
- Cancer-associated SPOP mutations enlarge nuclear size and facilitate nuclear envelope rupture upon farnesyltransferase inhibitor treatment.The Journal of clinical investigation · 2025Article
- Article
- Rise and SINE: roles of transcription factors and retrotransposons in zygotic genome activation.Nature reviews. Molecular cell biology · 2025Article
- How the chromatin landscape influences nuclear morphology.Frontiers in cell and developmental biology · 2025Review
- Review
- Cell state transitions are decoupled from cell division during early embryo development.Nature cell biology · 2024Article
- A deep learning-based toolkit for 3D nuclei segmentation and quantitative analysis in cellular and tissue context.Development (Cambridge, England) · 2024Article
- Temporospatial hierarchy and allele-specific expression of zygotic genome activation revealed by distant interspecific urochordate hybrids.Nature communications · 2024Article
- Manipulation of Embryonic Cleavage Geometry Using Magnetic Tweezers.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Collective effects of cell cleavage dynamics.Frontiers in cell and developmental biology · 2024Review
- Nuclear growth and import can be uncoupled.Molecular biology of the cell · 2024Article
- Principles of genome activation in the early embryo.Current opinion in genetics & development · 2023Review
- Article
- Actin filaments accumulated in the nucleus remain in the vicinity of condensing chromosomes in the zebrafish early embryo.Biology open · 2023Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
Abstract
Early Xenopus laevis embryogenesis is a robust system for investigating mechanisms of developmental timing. After a series of rapid cell divisions with concomitant reductions in cell size, the first major developmental transition is the midblastula transition (MBT), when zygotic transcription begins and cell cycles elongate. Whereas the maintenance of a constant nuclear-to-cytoplasmic (N/C) volume ratio is a conserved cellular property, it has long been recognized that the N/C volume ratio changes dramatically during early Xenopus development. We investigated how changes in nuclear size and the N/C volume ratio during early development contribute to the regulation of MBT timing. Whereas previous studies suggested a role for the N/C volume ratio in MBT timing, none directly tested the effects of altering nuclear size. In this study, we first quantify blastomere and nuclear sizes in X. laevis embryos, demonstrating that the N/C volume ratio increases prior to the MBT. We then manipulate nuclear volume in embryos by microinjecting different nuclear scaling factors, including import proteins, lamins, and reticulons. Using this approach, we show that increasing the N/C volume ratio in pre-MBT embryos leads to premature activation of zygotic gene transcription and early onset of longer cell cycles. Conversely, decreasing the N/C volume ratio delays zygotic transcription and leads to additional rapid cell divisions. Whereas the DNA-to-cytoplasmic ratio has been implicated in MBT timing, our data show that nuclear size also contributes to the regulation of MBT timing, demonstrating the functional significance of nuclear size during development.
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