ArticleChemistry & biology2014
Peptide macrocyclization catalyzed by a prolyl oligopeptidase involved in α-amanitin biosynthesis.
Article in Chemistry & biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 114 citations in OpenAlex.
- The "dark magic mushroom" co-produces amatoxins and psilocybin.bioRxiv : the preprint server for biology · 2026Article
- Convergence and parallelism in the evolution of plant metabolism.Journal of integrative plant biology · 2026Review
- Myxarylin: TotalJournal of the American Chemical Society · 2026Article
- Leaderless RiPPs expand the repertoire of fungal secondary metabolites.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Identification of a key peptide cyclase for novel cyclic peptide discovery in Pseudostellaria heterophylla.Plant communications · 2025Article
- Pangenomics of the death cap mushroom Amanita phalloides, and of Agaricales, reveals dynamic evolution of toxin genes in an invasive range.The ISME journal · 2023Article
- Exploring the Limits of Cyanobactin Macrocyclase PatGmac: Cyclization of PawS-Derived Peptide Sunflower Trypsin Inhibitor-1 and Cyclotide Kalata B1.Journal of natural products · 2023Article
- Emulating nonribosomal peptides with ribosomal biosynthetic strategies.RSC chemical biology · 2023Review
- Structural and Functional Analysis of Peptides Derived from KEX2-Processed Repeat Proteins in Agaricomycetes Using Reverse Genetics and Peptidomics.Microbiology spectrum · 2022Article
- The occurrence of ansamers in the synthesis of cyclic peptides.Nature communications · 2022Article
- Exploring fungal RiPPs from the perspective of chemical ecology.Fungal biology and biotechnology · 2022Review
- Subcellular localization of fungal specialized metabolites.Fungal biology and biotechnology · 2022Review
- Post-Proline Cleaving Enzymes (PPCEs): Classification, Structure, Molecular Properties, and Applications.Plants (Basel, Switzerland) · 2022Review
- Genes and evolutionary fates of the amanitin biosynthesis pathway in poisonous mushrooms.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Strategy to Select Macrocyclic Peptides Featuring Asymmetric Molecular Scaffolds as Cyclization Units by Phage Display.Journal of the American Chemical Society · 2022Article
- Enzymatic Synthesis Assisted Discovery of Proline-Rich Macrocyclic Peptides in Marine Sponges.Chembiochem : a European journal of chemical biology · 2021Article
- Review
- Differential Expression of Amanitin Biosynthetic Genes and Novel Cyclic Peptides inJournal of fungi (Basel, Switzerland) · 2021Article
- Novel Cyclic Peptides from LethalJournal of fungi (Basel, Switzerland) · 2021Article
- New developments in RiPP discovery, enzymology and engineering.Natural product reports · 2021Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Amatoxins are ribosomally encoded and posttranslationally modified peptides that account for the majority of fatal mushroom poisonings of humans. A representative amatoxin is the bicyclic octapeptide α-amanitin, formed via head-to-tail macrocyclization, which is ribosomally biosynthesized as a 35-amino acid propeptide in Amanita bisporigera and in the distantly related mushroom Galerina marginata. Although members of the prolyl oligopeptidase (POP) family of serine proteases have been proposed to play a role in α-amanitin posttranslational processing, the exact mechanistic details are not known. Here, we show that a specific POP (GmPOPB) is required for toxin maturation in G. marginata. Recombinant GmPOPB catalyzed two nonprocessive reactions: hydrolysis at an internal Pro to release the C-terminal 25-mer from the 35-mer propeptide and transpeptidation at the second Pro to produce the cyclic octamer. On the other hand, we show that GmPOPA, the putative housekeeping POP of G. marginata, behaves like a conventional POP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.