ArticleScientific reports2014
Development of a general method for detection and quantification of the P35S promoter based on assessment of existing methods.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A comparative analysis of stably expressed genes across diverse angiosperms exposes flexibility in underlying promoter architecture.G3 (Bethesda, Md.) · 2023Article
- A comparative analysis of stably expressed genes across diverse angiosperms exposes flexibility in underlying promoter architecture.bioRxiv : the preprint server for biology · 2023Article
- An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a.Foods (Basel, Switzerland) · 2023Article
- An accurate, reliable, and universal qPCR method to identify homozygous single insert T-DNA with the example of transgenic rice.Frontiers in plant science · 2023Article
- Design and Analysis of Native Photorespiration Gene Motifs of Promoter Untranslated Region Combinations Under Short Term Abiotic Stress Conditions.Frontiers in plant science · 2022Article
- Selected Instrumental Techniques Applied in Food and Feed: Quality, Safety and Adulteration Analysis.Foods (Basel, Switzerland) · 2021Review
- Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection.BMC biotechnology · 2019Article
- Highly sensitive immunosensing platform for one-step detection of genetically modified crops.Scientific reports · 2019Article
- Lack of specificity associated with using molecular beacons in loop mediated amplification assays.BMC biotechnology · 2019Article
- Targeted Next Generation Sequencing to study insert stability in genetically modified plants.Scientific reports · 2019Article
- Optimised LAMP allows single copy detection of 35Sp and NOSt in transgenic maize using Bioluminescent Assay in Real Time (BART).Scientific reports · 2018Article
- Review
- A Quantitative PCR-Electrochemical Genosensor Test for the Screening of Biotech Crops.Sensors (Basel, Switzerland) · 2017Article
- A highly sensitive and specific method for the screening detection of genetically modified organisms based on digital PCR without pretreatment.Scientific reports · 2015Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cauliflower mosaic virus (CaMV) 35S promoter (P35S) is a commonly used target for detection of genetically modified organisms (GMOs). There are currently 24 reported detection methods, targeting different regions of the P35S promoter. Initial assessment revealed that due to the absence of primer binding sites in the P35S sequence, 19 of the 24 reported methods failed to detect P35S in MON88913 cotton, and the other two methods could only be applied to certain GMOs. The rest three reported methods were not suitable for measurement of P35S in some testing events, because SNPs in binding sites of the primer/probe would result in abnormal amplification plots and poor linear regression parameters. In this study, we discovered a conserved region in the P35S sequence through sequencing of P35S promoters from multiple transgenic events, and developed new qualitative and quantitative detection systems targeting this conserved region. The qualitative PCR could detect the P35S promoter in 23 unique GMO events with high specificity and sensitivity. The quantitative method was suitable for measurement of P35S promoter, exhibiting good agreement between the amount of template and Ct values for each testing event. This study provides a general P35S screening method, with greater coverage than existing methods.
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