ArticleIslets2014
Optogenetic control of insulin secretion in intact pancreatic islets with β-cell-specific expression of Channelrhodopsin-2.
Article in Islets, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 64 citations in OpenAlex.
- Microbubble-enhanced ultrasound stimulation of β-cells improves insulin release and glycemic control in mice.Journal of nanobiotechnology · 2025Article
- Efficient transduction of pancreas tissue slices with genetically encoded calcium integrators.bioRxiv : the preprint server for biology · 2025Article
- Optogenetics in Pancreatic Islets: Actuators and Effects.Diabetes · 2024Review
- Optogenetic therapeutic strategies for diabetes mellitus.Journal of diabetes · 2024Review
- Light-stimulated insulin secretion from pancreatic islet-like organoids derived from human pluripotent stem cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Dynamic changes in β-cell [CaMolecular metabolism · 2022Article
- Deciphering Molecular Mechanisms and Intervening in Physiological and Pathophysiological Processes of CaCells · 2021Review
- State-of-the-art microscopy to understand islets of Langerhans: what to expect next?Immunology and cell biology · 2021Review
- Wireless optogenetics protects against obesity via stimulation of non-canonical fat thermogenesis.Nature communications · 2020Article
- Leader β-cells coordinate CaNature metabolism · 2019Article
- Sub-millisecond Control of Neuronal Firing by Organic Light-Emitting Diodes.Frontiers in bioengineering and biotechnology · 2019Article
- Optical tools for understanding the complexity of β-cell signalling and insulin release.Nature reviews. Endocrinology · 2018Review
- Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis.Lab on a chip · 2018Article
- The role of beta cell heterogeneity in islet function and insulin release.Journal of molecular endocrinology · 2018Review
- Defining the ionic mechanisms of optogenetic control of vascular tone by channelrhodopsin-2.British journal of pharmacology · 2018Article
- Review
- Article
- δ-cells and β-cells are electrically coupled and regulate α-cell activity via somatostatin.The Journal of physiology · 2018Article
- Spatially Organized β-Cell Subpopulations Control Electrical Dynamics across Islets of Langerhans.Biophysical journal · 2017Article
- Optogenetic regulation of insulin secretion in pancreatic β-cells.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insulin is secreted from the pancreatic β-cells in response to elevated glucose. In intact islets the capacity for insulin release is determined by a complex interplay between different cell types. This has made it difficult to specifically assess the role of β-cell defects to the insulin secretory impairment in type 2 diabetes. Here we describe a new approach, based on optogenetics, that enables specific investigation of β-cells in intact islets. We used transgenic mice expressing the light-sensitive cation channel Channelrhodopsin-2 (ChR2) under control of the insulin promoter. Glucose tolerance in vivo was assessed using intraperitoneal glucose tolerance tests, and glucose-induced insulin release was measured from static batch incubations. ChR2 localization was determined by fluorescence confocal microscopy. The effect of ChR2 stimulation with blue LED light was assessed using Ca(2+) imaging and static islet incubations. Light stimulation of islets from transgenic ChR2 mice triggered prompt increases in intracellular Ca(2+). Moreover, light stimulation enhanced insulin secretion in batch-incubated islets at low and intermediate but not at high glucose concentrations. Glucagon release was not affected. Beta-cells from mice rendered diabetic on a high-fat diet exhibited a 3.5-fold increase in light-induced Ca(2+) influx compared with mice on a control diet. Furthermore, light enhanced insulin release also at high glucose in these mice, suggesting that high-fat feeding leads to a compensatory potentiation of the Ca(2+) response in β-cells. The results demonstrate the usefulness and versatility of optogenetics for studying mechanisms of perturbed hormone secretion in diabetes with high time-resolution and cell-specificity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.