Evidence map›Paper›PMID 25483735›Full record

ArticleThe Journal of surgical research2015

BH3-only proteins contribute to steatotic liver ischemia-reperfusion injury.

Bernard J DuBray, Kendra D Conzen, Gundumi A Upadhya, Kristen L Gunter, Jianluo Jia, Brett L Knolhoff, Thallachallour Mohanakumar, William C Chapman, Christopher D Anderson

Abstract read
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In one paragraph

Article in The Journal of surgical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. International journal of molecular sciences · 2020
    Article
  4. Article
  5. Inflammasome activation and function in liver disease.Nature reviews. Gastroenterology & hepatology · 2015
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Bernard J DuBrayDepartment of Surgery, Washington University, Saint Louis, Missouri.
Kendra D ConzenDepartment of Surgery, Washington University, Saint Louis, Missouri.
Gundumi A UpadhyaDepartment of Surgery, Washington University, Saint Louis, Missouri.
Kristen L GunterDepartment of Surgery, Washington University, Saint Louis, Missouri.
Jianluo JiaDepartment of Surgery, Washington University, Saint Louis, Missouri.
Brett L KnolhoffDepartment of Surgery, Washington University, Saint Louis, Missouri.
Thallachallour MohanakumarDepartment of Surgery, Washington University, Saint Louis, Missouri.
William C ChapmanDepartment of Surgery, Washington University, Saint Louis, Missouri.
Christopher D AndersonDepartment of Surgery, University of Mississippi Medical Center, Jackson, Mississippi. Electronic address: cdanderson@umc.edu.

Funding

Washington University Nutrition Obesity Research CenterP30DK056341 · NIDDK · WASHINGTON UNIVERSITY · PI Brian N Finck · 1999 to 2026
$30.2M
HEPATIC STEATOSIS AND ENDOPLASMIC RETICULUM STRESS IN LIVER ALLOGRAFT INJURYR21DK090661 · NIDDK · UNIVERSITY OF MISSISSIPPI MED CTR · PI ANDERSON, CHRISTOPHER DANIEL · 2011 to 2012
$406k
NIDDK NIH HHS L30 DK082350NIDDK NIH HHS P30 DK056341NIDDK NIH HHS R21 DK090661
6 · The paper itself

Abstract

backgroundIschemia-reperfusion injury (IRI) to the liver continues to be a source of significant morbidity, especially in patients with hepatic steatosis. This is a growing problem given the increase in nonalcoholic fatty liver disease. B-cell lymphoma-2 homology3-only members of the B-cell lymphoma-2 protein family are known mediators of cellular apoptosis, although their role in hepatic IRI is still emerging. The goal of this study was to investigate the effect of Bim and Bid on warm hepatic IRI in the setting of steatosis.

methodsLean and obese Bim and/or Bid wild-type (WT) and double knockout (DKO) mice underwent 60 min of warm hepatic ischemia using a 70% segmental occlusion technique. Obesity and hepatic steatosis were induced using a high fat diet. Hepatocellular injury patterns were compared among lean and steatotic mice after reperfusion. Differences were analyzed using a Student t-test and reported as mean ± standard error of the mean.

resultsDKO mice were protected from IRI relative to WT. A high fat diet created equal degrees of steatosis in both WT and DKO mice. The IRI was increased in steatotic WT livers; however, DKO mice remained protected relative to WT despite hepatic steatosis.

conclusionsThe B-cell lymphoma-2 homology3-only proteins are important mediators of hepatic IRI in both lean and steatotic livers. These mechanisms have been underappreciated in steatotic liver injury and may be leveraged as targets for intervention in clinical scenarios such as transplant and hypovolemic shock.

Indexed as

AnimalsApoptosis Regulatory ProteinsBcl-2-Like Protein 11BH3 Interacting Domain Death Agonist ProteinFatty LiverLiverMembrane ProteinsMice, KnockoutProto-Oncogene ProteinsReperfusion InjuryApoptosis Regulatory ProteinsBcl2l11 protein, mouseBcl-2-Like Protein 11BH3 Interacting Domain Death Agonist ProteinBid protein, mouseMembrane ProteinsProto-Oncogene ProteinsApoptosisBCL-2 proteinsIschemia–reperfusionSteatosis

Identifiers

PMID25483735
PMCPMC4369674

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.