ArticleCell cycle (Georgetown, Tex.)2014
Identification of DNA methylation changes at cis-regulatory elements during early steps of HSC differentiation using tagmentation-based whole genome bisulfite sequencing.
Article in Cell cycle (Georgetown, Tex.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 58 citations in OpenAlex.
- Hematopoietic stem cell metabolism within the bone marrow niche - insights and opportunities.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Exploring the genetic and epigenetic origins of juvenile myelomonocytic leukemia using newborn screening samples.Leukemia · 2022Article
- Epigenetic signatures in cancer: proper controls, current challenges and the potential for clinical translation.Genome medicine · 2021Review
- DNA Methylation in T-Cell Acute Lymphoblastic Leukemia: In Search for Clinical and Biological Meaning.International journal of molecular sciences · 2021Review
- Regulation of the methylome in differentiation from adult stem cells may underpin vitamin D risk in MS.Genes and immunity · 2020Article
- The DNA methylation landscape of hematological malignancies: an update.Molecular oncology · 2020Review
- Allele-specific DNA methylation is increased in cancers and its dense mapping in normal plus neoplastic cells increases the yield of disease-associated regulatory SNPs.Genome biology · 2020Article
- Methylome-based cell-of-origin modeling (Methyl-COOM) identifies aberrant expression of immune regulatory molecules in CLL.Genome medicine · 2020Article
- The transcriptomic and epigenetic map of vascular quiescence in the continuous lung endothelium.eLife · 2018Article
- Exposure to the gut microbiota drives distinct methylome and transcriptome changes in intestinal epithelial cells during postnatal development.Genome medicine · 2018Article
- Sexual-lineage-specific DNA methylation regulates meiosis in Arabidopsis.Nature genetics · 2018Article
- Genome-wide DNA methylation is predictive of outcome in juvenile myelomonocytic leukemia.Nature communications · 2017Observational
- RAS-pathway mutation patterns define epigenetic subclasses in juvenile myelomonocytic leukemia.Nature communications · 2017Article
- How nutrition and the maternal microbiota shape the neonatal immune system.Nature reviews. Immunology · 2017Review
- Understanding the genetic liability to schizophrenia through the neuroepigenome.Schizophrenia research · 2016Review
- Unperturbed vs. post-transplantation hematopoiesis: both in vivo but different.Current opinion in hematology · 2016Review
- Assessing DNA methylation in the developing human intestinal epithelium: potential link to inflammatory bowel disease.Mucosal immunology · 2016Article
- Epigenetic silencing of AKAP12 in juvenile myelomonocytic leukemia.Epigenetics · 2016Article
- Second German-Catalan workshop on epigenetics & cancer.Epigenetics · 2015Article
- Dynamic DNA methylation discovered during HSC differentiation.Cell cycle (Georgetown, Tex.) · 2015Article
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Authors and funding
20 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epigenetic alterations during cellular differentiation are a key molecular mechanism which both instructs and reinforces the process of lineage commitment. Within the haematopoietic system, progressive changes in the DNA methylome of haematopoietic stem cells (HSCs) are essential for the effective production of mature blood cells. Inhibition or loss of function of the cellular DNA methylation machinery has been shown to lead to a severe perturbation in blood production and is also an important driver of malignant transformation. HSCs constitute a very rare cell population in the bone marrow, capable of life-long self-renewal and multi-lineage differentiation. The low abundance of HSCs has been a major technological barrier to the global analysis of the CpG methylation status within both HSCs and their immediate progeny, the multipotent progenitors (MPPs). Within this Extra View article, we review the current understanding of how the DNA methylome regulates normal and malignant hematopoiesis. We also discuss the current methodologies that are available for interrogating the DNA methylation status of HSCs and MPPs and describe a new data set that was generated using tagmentation-based whole genome bisulfite sequencing (TWGBS) in order to comprehensively map methylated cytosines using the limited amount of genomic DNA that can be harvested from rare cell populations. Extended analysis of this data set clearly demonstrates the added value of genome-wide sequencing of methylated cytosines and identifies novel important cis-acting regulatory regions that are dynamically remodeled during the first steps of haematopoietic differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.