Evidence map›Paper›PMID 25482782›Full record

ArticlePlant signaling & behavior2014

Intronic T-DNA insertion in Arabidopsis NBR1 conditionally affects wild-type transcript level.

Milagros Collados Rodríguez, Anna Wawrzyńska, Agnieszka Sirko

Open access · hybridAbstract read
In one paragraph

Article in Plant signaling & behavior, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 11 citations in OpenAlex.

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  9. Allele-Specific Interactions betweenFrontiers in plant science · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Milagros Collados Rodrígueza Institute of Biochemistry and Biophysics ; Polish Academy of Sciences ; Warsaw , Poland.
Anna Wawrzyńska
Agnieszka Sirko
Polish Academy of Sciences · PLInstitute of Biochemistry and Biophysics, Polish Academy of Sciences · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The SALK_135513 line of Arabidopsis thaliana is annotated by GenBank to have the T-DNA insertion in the fourth exon of NBR1 (At4g24690). Careful molecular analyses of the homozygous plants of SALK_135513 line indicated the place of T-DNA insertion in the fourth intron. Unexpectedly, 2 kinds of NBR1 transcripts, the wild-type and the mutated, resulting from alternative splicing events, were detected in those plants. Our findings explain the problems encountered by us with phenotypic evaluation of this line and emphasize the necessity for independent verification of the exact insertion site followed by careful expression studies when working with Arabidopsis T-DNA insertional mutants.

Indexed as

ArabidopsisArabidopsis ProteinsCarrier ProteinsDNA, BacterialGene Expression Regulation, PlantIntronsMutagenesis, InsertionalReal-Time Polymerase Chain ReactionRNA, MessengerRNA Splice SitesStress, PhysiologicalArabidopsis ProteinsCarrier ProteinsDNA, BacterialNBR1 protein, ArabidopsisRNA, MessengerRNA Splice SitesT-DNAarabidopsisAt4g24690intronic insertionKO, knock-outMV, methyl viologenNBR1NBR1, Neighbor of BRCA1qRT-PCR, quantitative RT-PCR.SALK_135513sqRT-PCR, semiquantitative RT-PCRT-DNA mutantT-DNA, transferred DNAtranscriptionWT – wild-type

Identifiers

PMID25482782
PMCPMC4622546
OpenAlexW2146421853

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.