ArticleCell reports2014
A distal locus element mediates IFN-γ priming of lipopolysaccharide-stimulated TNF gene expression.
Article in Cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
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- Genetic variants associated with psychiatric disorders are enriched at epigenetically active sites in lymphoid cells.Nature communications · 2022Article
- Trained innate immunity, long-lasting epigenetic modulation, and skewed myelopoiesis by heme.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- UPEC kidney infection triggers neuro-immune communication leading to modulation of local renal inflammation by splenic IFNγ.PLoS pathogens · 2021Article
- Identification of a Distal Locus Enhancer Element That Controls Cell Type-SpecificJournal of immunology (Baltimore, Md. : 1950) · 2020Article
- Frequent interferon regulatory factor 1 (IRF1) binding at remote elements without histone modification.The Journal of biological chemistry · 2018Article
- Shared genetic effects on chromatin and gene expression indicate a role for enhancer priming in immune response.Nature genetics · 2018Article
- Higher-Order Chromatin Regulation of Inflammatory Gene Expression.Mediators of inflammation · 2017Review
- Epigenetic Regulation of Myeloid Cells.Microbiology spectrum · 2016Review
- Sumoylation coordinates the repression of inflammatory and anti-viral gene-expression programs during innate sensing.Nature immunology · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Interferon γ (IFN-γ) priming sensitizes monocytes and macrophages to lipopolysaccharide (LPS) stimulation, resulting in augmented expression of a set of genes including TNF. Here, we demonstrate that IFN-γ priming of LPS-stimulated TNF transcription requires a distal TNF/LT locus element 8 kb upstream of the TNF transcription start site (hHS-8). IFN-γ stimulation leads to increased DNase I accessibility of hHS-8 and its recruitment of interferon regulatory factor 1 (IRF1), and subsequent LPS stimulation enhances H3K27 acetylation and induces enhancer RNA synthesis at hHS-8. Ablation of IRF1 or targeting the hHS-8 IRF1 binding site in vivo with Cas9 linked to the KRAB repressive domain abolishes IFN-γ priming, but does not affect LPS induction of the gene. Thus, IFN-γ poises a distal enhancer in the TNF/LT locus by chromatin remodeling and IRF1 recruitment, which then drives enhanced TNF gene expression in response to a secondary toll-like receptor (TLR) stimulus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.