ArticleInfluenza and other respiratory viruses2015
Culturing of respiratory viruses in well-differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses.
Article in Influenza and other respiratory viruses, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Detection of respiratory viruses directly from clinical samples using next-generation sequencing: A literature review of recent advances and potential for routine clinical use.Reviews in medical virology · 2022Pooled it
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- Drug-Free Nasal Spray as a Barrier against SARS-CoV-2 and Its Delta Variant: In Vitro Study of Safety and Efficacy in Human Nasal Airway Epithelia.International journal of molecular sciences · 2022Article
- Influenza virus infection increases ACE2 expression and shedding in human small airway epithelial cells.The European respiratory journal · 2021Article
- Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): a Systemic Infection.Clinical microbiology reviews · 2021Review
- Harness Organoid Models for Virological Studies in Animals: A Cross-Species Perspective.Frontiers in microbiology · 2021Review
- A Perspective on Organoids for Virology Research.Viruses · 2020Article
- Characterization and Treatment of SARS-CoV-2 in Nasal and Bronchial Human Airway Epithelia.Cell reports. Medicine · 2020Article
- Isolation and characterization of WUPyV in polarized human airway epithelial cells.BMC infectious diseases · 2020Article
- Viral Coinfection Replaces Effects of Suilysin on Streptococcus suis Adherence to and Invasion of Respiratory Epithelial Cells Grown under Air-Liquid Interface Conditions.Infection and immunity · 2019Article
- Anti-H7N9 avian influenza A virus activity of interferon in pseudostratified human airway epithelium cell cultures.Virology journal · 2019Article
- Antiviral drug screening by assessing epithelial functions and innate immune responses in human 3D airway epithelium model.Antiviral research · 2018Article
- Glucocorticoid Clearance and Metabolite Profiling in an In Vitro Human Airway Epithelium Lung Model.Drug metabolism and disposition: the biological fate of chemicals · 2016Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCurrently, virus discovery is mainly based on molecular techniques. Here, we propose a method that relies on virus culturing combined with state-of-the-art sequencing techniques. The most natural ex vivo culture system was used to enable replication of respiratory viruses.
methodThree respiratory clinical samples were tested on well-differentiated pseudostratified tracheobronchial human airway epithelial (HAE) cultures grown at an air-liquid interface, which resemble the airway epithelium. Cells were stained with convalescent serum of the patients to identify infected cells and apical washes were analyzed by VIDISCA-454, a next-generation sequencing virus discovery technique.
resultsInfected cells were observed for all three samples. Sequencing subsequently indicated that the cells were infected by either human coronavirus OC43, influenzavirus B, or influenzavirus A. The sequence reads covered a large part of the genome (52%, 82%, and 57%, respectively).
conclusionWe present here a new method for virus discovery that requires a virus culture on primary cells and an antibody detection. The virus in the harvest can be used to characterize the viral genome sequence and cell tropism, but also provides progeny virus to initiate experiments to fulfill the Koch's postulates.
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