ArticleNucleus (Austin, Tex.)
Single molecule localization microscopy of the distribution of chromatin using Hoechst and DAPI fluorescent probes.
Article in Nucleus (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- 'In silico labelling': Exploring the biological landscape at the nanoscale using intelligent multimodal optical microscopy.Journal of microscopy · 2026Review
- Molecular engineering of Cyanine-Hoechst hybrid materials for nanoscale DNA and chromatin imaging.JPhys materials · 2026Article
- Luminescence Properties of Hoechst 33258 in Polyvinyl Alcohol Films.International journal of molecular sciences · 2025Article
- Precisely modulating the chromatin trackerChemical science · 2024Article
- Expansion microscopy allows high resolution single cell analysis of epigenetic readers.Nucleic acids research · 2022Article
- Study on Long-Term Tracing of Fibroblasts on Three-Dimensional Tissue Engineering Scaffolds Based on Graphene Quantum Dots.International journal of molecular sciences · 2022Article
- Viro-fluidics: Real-time analysis of virus production kinetics at the single-cell level.Biophysical reports · 2022Article
- Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe.Science advances · 2022Article
- HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein.Nucleic acids research · 2022Article
- 3-Pyridinylidene Derivatives of Chemically Modified Lupane and Ursane Triterpenes as Promising Anticancer Agents by Targeting Apoptosis.International journal of molecular sciences · 2021Article
- Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.Journal of fluorescence · 2021Article
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Cryo-nanoscale chromosome imaging-future prospects.Biophysical reviews · 2020Review
- Ferrostatin-1 protects HT-22 cells from oxidative toxicity.Neural regeneration research · 2020Article
- Colorimetric and Fluorimetric DNA Detection with a Hydroxystyryl-Quinolizinium Photoacid and Its Application for Cell Imaging.Chemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
- A guide to visualizing the spatial epigenome with super-resolution microscopy.The FEBS journal · 2019Review
- Correlative single-molecule localization microscopy and electron tomography reveals endosome nanoscale domains.Traffic (Copenhagen, Denmark) · 2019Article
- Super-Resolution Microscopy of Chromatin.Genes · 2019Review
- Rhodamine-Hoechst positional isomers for highly efficient staining of heterochromatin.Chemical science · 2019Article
- PML-like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication-based single-strand breaks.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several approaches have been described to fluorescently label and image DNA and chromatin in situ on the single-molecule level. These superresolution microscopy techniques are based on detecting optically isolated, fluorescently tagged anti-histone antibodies, fluorescently labeled DNA precursor analogs, or fluorescent dyes bound to DNA. Presently they suffer from various drawbacks such as low labeling efficiency or interference with DNA structure. In this report, we demonstrate that DNA minor groove binding dyes, such as Hoechst 33258, Hoechst 33342, and DAPI, can be effectively employed in single molecule localization microscopy (SMLM) with high optical and structural resolution. Upon illumination with low intensity 405 nm light, a small subpopulation of these molecules stochastically undergoes photoconversion from the original blue-emitting form to a green-emitting form. Using a 491 nm laser excitation, fluorescence of these green-emitting, optically isolated molecules was registered until "bleached". This procedure facilitated substantially the optical isolation and localization of large numbers of individual dye molecules bound to DNA in situ, in nuclei of fixed mammalian cells, or in mitotic chromosomes, and enabled the reconstruction of high-quality DNA density maps. We anticipate that this approach will provide new insights into DNA replication, DNA repair, gene transcription, and other nuclear processes.
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