ArticleNucleus (Austin, Tex.)2014
Reprogramming of fibroblast nuclei in cloned bovine embryos involves major structural remodeling with both striking similarities and differences to nuclear phenotypes of in vitro fertilized embryos.
Article in Nucleus (Austin, Tex.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Mechanisms underlying low mutation rates in mammalian oocytes and preimplantation embryos.Nucleic acids research · 2025Review
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- Balance of osmotic pressures determines the nuclear-to-cytoplasmic volume ratio of the cell.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Evaluation of chromatin mesoscale organization.APL bioengineering · 2022Article
- Alterations to Genome Organisation in Stem Cells, Their Differentiation and Associated Diseases.Results and problems in cell differentiation · 2022Article
- Live imaging of chromatin distribution reveals novel principles of nuclear architecture and chromatin compartmentalization.Science advances · 2021Article
- Article
- Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How.International journal of molecular sciences · 2020Review
- Three-dimensional analysis of nuclear heterochromatin distribution during early development in the rabbit.Chromosoma · 2018Article
- Transcription-dependent radial distribution of TCF7L2 regulated genes in chromosome territories.Chromosoma · 2017Article
- Initial high-resolution microscopic mapping of active and inactive regulatory sequences proves non-random 3D arrangements in chromatin domain clusters.Epigenetics & chromatin · 2017Article
- Imaging chromatin nanostructure with binding-activated localization microscopy based on DNA structure fluctuations.Nucleic acids research · 2017Article
- The Molecular Revolution in Cutaneous Biology: Chromosomal Territories, Higher-Order Chromatin Remodeling, and the Control of Gene Expression in Keratinocytes.The Journal of investigative dermatology · 2017Review
- Nuclear morphologies: their diversity and functional relevance.Chromosoma · 2017Review
- Stage-dependent remodeling of the nuclear envelope and lamina during rabbit early embryonic development.The Journal of reproduction and development · 2016Article
- Building up the nucleus: nuclear organization in the establishment of totipotency and pluripotency during mammalian development.Genes & development · 2016Review
- 3D structured illumination microscopy of mammalian embryos and spermatozoa.BMC developmental biology · 2015Article
- A transient ischemic environment induces reversible compaction of chromatin.Genome biology · 2015Article
- Remodeling of the Nuclear Envelope and Lamina during Bovine Preimplantation Development and Its Functional Implications.PloS one · 2015Article
- Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments.Epigenetics & chromatin · 2015Article
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear landscapes were studied during preimplantation development of bovine embryos, generated either by in vitro fertilization (IVF), or generated as cloned embryos by somatic cell nuclear transfer (SCNT) of bovine fetal fibroblasts, using 3-dimensional confocal laser scanning microscopy (3D-CLSM) and structured illumination microscopy (3D-SIM). Nuclear landscapes of IVF and SCNT embryonic nuclei were compared with each other and with fibroblast nuclei. We demonstrate that reprogramming of fibroblast nuclei in cloned embryos requires changes of their landscapes similar to nuclei of IVF embryos. On the way toward the 8-cell stage, where major genome activation occurs, a major lacuna, enriched with splicing factors, was formed in the nuclear interior and chromosome territories (CTs) were shifted toward the nuclear periphery. During further development the major lacuna disappeared and CTs were redistributed throughout the nuclear interior forming a contiguous higher order chromatin network. At all stages of development CTs of IVF and SCNT embryonic nuclei were built up from chromatin domain clusters (CDCs) pervaded by interchromatin compartment (IC) channels. Quantitative analyses revealed a highly significant enrichment of RNA polymerase II and H3K4me3, a marker for transcriptionally competent chromatin, at the periphery of CDCs. In contrast, H3K9me3, a marker for silent chromatin, was enriched in the more compacted interior of CDCs. Despite these striking similarities, we also detected major differences between nuclear landscapes of IVF and cloned embryos. Possible implications of these differences for the developmental potential of cloned animals remain to be investigated. We present a model, which integrates generally applicable structural and functional features of the nuclear landscape.
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