ArticleProceedings of the National Academy of Sciences of the United States of America1989
Actin polymerization induces a shape change in actin-containing vesicles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 129 citations in OpenAlex.
- A unified purification method for actin-binding proteins using a TEV-cleavable His-Strep-tag.MethodsX · 2024Article
- Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many.ACS synthetic biology · 2023Review
- Liposome Deformation Induced by Membrane-Binding Peptides.Micromachines · 2023Article
- Shaping Liposomes by Cell-Free Expressed Bacterial Microtubules.ACS synthetic biology · 2021Article
- Ventral stress fibers induce plasma membrane deformation in human fibroblasts.Molecular biology of the cell · 2021Article
- Systems biology of cellular membranes: a convergence with biophysics.Wiley interdisciplinary reviews. Systems biology and medicine · 2017Review
- Toward the reconstitution of synthetic cell motility.Cell adhesion & migration · 2016Review
- Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch.Nature cell biology · 2015Review
- Effect of Osmotic Pressure on the Stability of Whole Inactivated Influenza Vaccine for Coating on Microneedles.PloS one · 2015Article
- Model systems for studying cell adhesion and biomimetic actin networks.Beilstein journal of nanotechnology · 2014Review
- Epithelial machines of morphogenesis and their potential application in organ assembly and tissue engineering.Biomechanics and modeling in mechanobiology · 2012Review
- Transformation of actoHMM assembly confined in cell-sized liposome.Langmuir : the ACS journal of surfaces and colloids · 2011Article
- Bacillus subtilis MreB orthologs self-organize into filamentous structures underneath the cell membrane in a heterologous cell system.PloS one · 2011Article
- Article
- Biology under construction: in vitro reconstitution of cellular function.Nature reviews. Molecular cell biology · 2009Review
- Light-induced self-assembly of nanofibers inside liposomes.Soft matter · 2008Article
- Get round and stiff for mitosis.HFSP journal · 2008Article
- Analysis of actin dynamics at the leading edge of crawling cells: implications for the shape of keratocyte lamellipodia.European biophysics journal : EBJ · 2003Article
- Atomic force microscopy and light scattering of small unilamellar actin-containing liposomes.Biophysical journal · 2003Article
- Probing the cell peripheral movements by optical trapping technique.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have encapsulated actin filaments in the presence and absence of various actin-binding proteins into lipid vesicles. These vesicles are approximately the same size as animal cells and can be characterized by the same optical microscopic and mechanical techniques used to study cells. We demonstrate that the initially spherical vesicles can be forced into asymmetric, irregular shapes by polymerization of the actin that they contain. Deformation of the vesicles requires that the actin filaments be on average at least approximately 0.5 micron long as shown by the effects of gelsolin, an actin filament-nucleating protein. Filamin, a filament-crosslinking protein, caused the surfaces of the vesicles to have a smoother appearance. Heterogeneous distribution of actin filaments within the vesicles is caused by interfilament interactions and modulated by gelsolin and filamin. The vesicles provide a model system to study control of cell shape and cytoskeletal organization, membrane-cytoskeleton interactions, and cytomechanics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.