ArticleBMC genomics2014
Identification of a lineage specific zinc responsive genomic island in Mycobacterium avium ssp. paratuberculosis.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- The accessory role of the outer membrane porin protein MspD inMicrobiology (Reading, England) · 2026Article
- Binding of MAP3773c Protein ofInternational journal of molecular sciences · 2024Article
- The Fur-like regulatory protein MAP3773c modulates key metabolic pathways in Mycobacterium avium subsp. paratuberculosis under in-vitro iron starvation.Scientific reports · 2024Article
- Vibrio cholerae's mysterious Seventh Pandemic island (VSP-II) encodes novel Zur-regulated zinc starvation genes involved in chemotaxis and cell congregation.PLoS genetics · 2021Article
- Identification and Characterization ofJournal of bacteriology · 2021Article
- Nutritional immunity: the impact of metals on lung immune cells and the airway microbiome during chronic respiratory disease.Respiratory research · 2021Review
- Zinc: Multidimensional Effects on Living Organisms.Biomedicines · 2021Review
- Complete Genome Sequence of OvineMicroorganisms · 2020Article
- Article
- Elucidating the Regulon of a FurFrontiers in microbiology · 2020Article
- Bacterial zinc uptake regulator proteins and their regulons.Biochemical Society transactions · 2018Review
- Zinc depletion induces ribosome hibernation in mycobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Iron Acquisition in Mycobacterium avium subsp. paratuberculosis.Journal of bacteriology · 2015Article
- FurA contributes to the oxidative stress response regulation of Mycobacterium avium ssp. paratuberculosis.Frontiers in microbiology · 2015Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMaintenance of metal homeostasis is crucial in bacterial pathogenicity as metal starvation is the most important mechanism in the nutritional immunity strategy of host cells. Thus, pathogenic bacteria have evolved sensitive metal scavenging systems to overcome this particular host defence mechanism. The ruminant pathogen Mycobacterium avium ssp. paratuberculosis (MAP) displays a unique gut tropism and causes a chronic progressive intestinal inflammation. MAP possesses eight conserved lineage specific large sequence polymorphisms (LSP), which distinguish MAP from its ancestral M. avium ssp. hominissuis or other M. avium subspecies. LSP14 and LSP15 harbour many genes proposed to be involved in metal homeostasis and have been suggested to substitute for a MAP specific, impaired mycobactin synthesis.
resultsIn the present study, we found that a LSP14 located putative IrtAB-like iron transporter encoded by mptABC was induced by zinc but not by iron starvation. Heterologous reporter gene assays with the lacZ gene under control of the mptABC promoter in M. smegmatis (MSMEG) and in a MSMEG∆furB deletion mutant revealed a zinc dependent, metalloregulator FurB mediated expression of mptABC via a conserved mycobacterial FurB recognition site. Deep sequencing of RNA from MAP cultures treated with the zinc chelator TPEN revealed that 70 genes responded to zinc limitation. Remarkably, 45 of these genes were located on a large genomic island of approximately 90 kb which harboured LSP14 and LSP15. Thirty-five of these genes were predicted to be controlled by FurB, due to the presence of putative binding sites. This clustering of zinc responsive genes was exclusively found in MAP and not in other mycobacteria.
conclusionsOur data revealed a particular genomic signature for MAP given by a unique zinc specific locus, thereby suggesting an exceptional relevance of zinc for the metabolism of MAP. MAP seems to be well adapted to maintain zinc homeostasis which might contribute to the peculiarity of MAP pathogenicity.
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