ArticleThe Journal of biological chemistry2015
In vivo kinetics of formate metabolism in folate-deficient and folate-replete rats.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.Cellular and molecular life sciences : CMLS · 2022Article
- Ketogenic Diet Consumption Inhibited Mitochondrial One-Carbon Metabolism.International journal of molecular sciences · 2022Article
- Glutathione levels are associated with methotrexate resistance in acute lymphoblastic leukemia cell lines.Frontiers in oncology · 2022Article
- Systematic evaluation of urinary formic acid as a new potential biomarker for Alzheimer's disease.Frontiers in aging neuroscience · 2022Article
- Folinate Supplementation Ameliorates Methotrexate Induced Mitochondrial Formate Depletion In Vitro and In Vivo.International journal of molecular sciences · 2021Article
- Tracing Metabolic Fate of Mitochondrial Glycine Cleavage System Derived Formate In Vitro and In Vivo.International journal of molecular sciences · 2020Article
- Formate metabolism in health and disease.Molecular metabolism · 2020Review
- Size-dependent tissue-specific biological effects of core-shell structured FeJournal of nanobiotechnology · 2019Article
- Rat liver folate metabolism can provide an independent functioning of associated metabolic pathways.Scientific reports · 2019Article
- Cell cycle regulation of folate-mediated one-carbon metabolism.Wiley interdisciplinary reviews. Systems biology and medicine · 2018Review
- Lifestyle, metabolite, and genetic determinants of formate concentrations in a cross-sectional study in young, healthy adults.The American journal of clinical nutrition · 2018Observational
- Plasma metabolite profiles in healthy women differ after intervention with supplemental folic acidJournal of nutritional science · 2018Article
- Metabolite signatures of doxorubicin induced toxicity in human induced pluripotent stem cell-derived cardiomyocytes.Amino acids · 2017Article
- Alcohol Dehydrogenase 5 Is a Source of Formate for De Novo Purine Biosynthesis in HepG2 Cells.The Journal of nutrition · 2017Article
- Riboflavin Deficiency in Rats Decreases de novo Formate Production but Does Not Affect Plasma Formate Concentration.The Journal of nutrition · 2017Article
- One carbon metabolism in pregnancy: Impact on maternal, fetal and neonatal health.Molecular and cellular endocrinology · 2016Review
- Serine one-carbon catabolism with formate overflow.Science advances · 2016Article
- Formate metabolism in fetal and neonatal sheep.American journal of physiology. Endocrinology and metabolism · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
It is now established that the mitochondrial production of formate is a major process in the endogenous generation of folate-linked one-carbon groups. We have developed an in vivo approach involving the constant infusion of [(13)C]formate until isotopic steady state is attained to measure the rate of endogenous formate production in rats fed on either a folate-replete or folate-deficient diet. Formate was produced at a rate of 76 μmol·h(-1)·100 g of body weight(-1) in the folate-replete rats, and this was decreased by 44% in folate-deficient rats. This decreased formate production was confirmed in isolated rat liver mitochondria where formate production from serine, the principal precursor of one-carbon groups, was decreased by 85%, although formate production from sarcosine and dimethylglycine (choline metabolites) was significantly increased. We attribute this unexpected result to the demonstrated production of formaldehyde by sarcosine dehydrogenase and dimethylglycine dehydrogenase from their respective substrates in the absence of tetrahydrofolate and subsequent formation of formate by formaldehyde dehydrogenase. Comparison of formate production with the ingestion of dietary formate precursors (serine, glycine, tryptophan, histidine, methionine, and choline) showed that ∼75% of these precursors were converted to formate, indicating that formate is a significant, although underappreciated end product of choline and amino acid oxidation. Ingestion of a high protein diet did not result in increased production of formate, suggesting a regulation of the conversion of these precursors at the mitochondrial level to formate.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.