ArticleApplied and environmental microbiology2015
Genome-wide identification of genes necessary for biofilm formation by nosocomial pathogen Stenotrophomonas maltophilia reveals that orphan response regulator FsnR is a critical modulator.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 45 citations in OpenAlex.
- Molecular Regulation of Biofilm Development inPathogens (Basel, Switzerland) · 2026Review
- Role of SMF-1 and cbl pili inBiofilm · 2025Article
- Alleviation of heavy metals chromium, cadmium and lead and plant growth promotion in Vigna radiata L. plant using isolated Pseudomonas geniculata.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Stenotrophomonas maltophilia uses a c-di-GMP module to sense the mammalian body temperature during infection.PLoS pathogens · 2024Article
- GAPDH-Silence Microsphere via Reprogramming Macrophage Metabolism and eradicating Bacteria for Diabetic infection bone regeneration.Journal of nanobiotechnology · 2024Article
- Characterisation of sRNAs enriched in outer membrane vesicles of pathogenicJournal of extracellular biology · 2024Article
- Review
- Relationship between antibiotic resistance with class 1 integron and SmeDEF efflux pump encoding genes in clinical isolates of Stenotrophomonas maltophilia.Journal of applied genetics · 2023Article
- A guide toFrontiers in cellular and infection microbiology · 2023Review
- Comparative genomics analysis ofFrontiers in cellular and infection microbiology · 2023Article
- Dual Regulatory Role Exerted by Cyclic Dimeric GMP To Control FsnR-Mediated Bacterial Swimming.mBio · 2022Article
- BDSF Is a Degradation-Prone Quorum-Sensing Signal Detected by the Histidine Kinase RpfC ofApplied and environmental microbiology · 2022Article
- Genome-wide analysis reveals the emergence of multidrug resistantFrontiers in microbiology · 2022Article
- Global transcriptome analysis ofMicrobial genomics · 2021Article
- Advances in the Microbiology of Stenotrophomonas maltophilia.Clinical microbiology reviews · 2021Article
- Phenotypic and Transcriptomic Analyses of Seven Clinical Stenotrophomonas maltophilia Isolates Identify a Small Set of Shared and Commonly Regulated Genes Involved in the Biofilm Lifestyle.Applied and environmental microbiology · 2020Article
- Stenotrophomonas maltophilia: An Emerging Pathogen of the Respiratory Tract.The American journal of case reports · 2020Article
- Antibacterial and Antivirulence Activity of Glucocorticoid PYED-1 againstAntibiotics (Basel, Switzerland) · 2020Article
- Analysis of HrpG regulons and HrpG-interacting proteins by ChIP-seq and affinity proteomics in Xanthomonas campestris.Molecular plant pathology · 2020Article
- Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stenotrophomonas maltophilia is a Gram-negative bacterial pathogen of increasing concern to human health. Most clinical isolates of S. maltophilia efficiently form biofilms on biotic and abiotic surfaces, making this bacterium resistant to a number of antibiotic treatments and therefore difficult to eliminate. To date, very few studies have investigated the molecular and regulatory mechanisms responsible for S. maltophilia biofilm formation. Here we constructed a random transposon insertion mutant library of S. maltophilia ATCC 13637 and screened 14,028 clones. A total of 46 nonredundant genes were identified. Mutants of these genes exhibited marked changes in biofilm formation, suggesting that multiple physiological pathways, including extracellular polysaccharide production, purine synthesis, transportation, and peptide and lipid synthesis, are involved in bacterial cell aggregation. Of these genes, 20 putatively contributed to flagellar biosynthesis, indicating a critical role for cell motility in S.maltophilia biofilm formation. Genetic and biochemical evidence demonstrated that an orphan response regulator, FsnR, activated transcription of at least two flagellum-associated operons by directly binding to their promoters. This regulatory protein plays a fundamental role in controlling flagellar assembly, cell motility, and biofilm formation. These results provide a genetic basis to systematically study biofilm formation of S. maltophilia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.