ArticleApplied and environmental microbiology2015
Bacteriophage resistance mechanisms in the fish pathogen Flavobacterium psychrophilum: linking genomic mutations to changes in bacterial virulence factors.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 99 citations in OpenAlex.
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- Phage resistance mutations in a marine bacterium impact biogeochemically relevant cellular processes.Nature microbiology · 2026Article
- From Isolation to Application: Designing a Multi-Target Phage Cocktail for Bivalve Safety.Microorganisms · 2025Article
- A machine learning approach to predict strain-specific phage-host interactions.Scientific reports · 2025Article
- Design of Lytic Phage Cocktails TargetingViruses · 2025Article
- Current Trends in Approaches to Prevent and Control Antimicrobial Resistance in Aquatic Veterinary Medicine.Pathogens (Basel, Switzerland) · 2025Review
- Methylation of foreign DNA overcomes the restriction barrier ofApplied and environmental microbiology · 2025Article
- Characterization and genomic analysis of a jumbo phage, PG216, with broad lytic activity against several Vibrio species.Archives of virology · 2025Article
- Characterization of an Indigenous Lytic Phage Targeting Multidrug-ResistantGut microbes reports · 2025Article
- Genetic and phenotypic diversity of Flavobacterium psychrophilum isolates from Czech salmonid fish farms.BMC microbiology · 2024Article
- Article
- Gliding motility proteins GldJ and SprB contribute toJournal of bacteriology · 2024Article
- Perturbation of Quorum Sensing after the Acquisition of Bacteriophage Resistance Could Contribute to Novel Traits inMicroorganisms · 2023Article
- Back to the Roots:Applied and environmental microbiology · 2023Article
- Development and Evaluation of Bacteriophage Cocktail to Eradicate Biofilms Formed by an Extensively Drug-Resistant (XDR)Viruses · 2023Article
- Identification of a novel type II-C Cas9 from the fish pathogenFrontiers in microbiology · 2023Article
- Transposon mutagenesis and genome sequencing identify two novel, tandem genes involved in the colony spreading ofFrontiers in cellular and infection microbiology · 2023Article
- Two functionally distinct heme/iron transport systems are virulence determinants of the fish pathogenVirulence · 2022Article
- Reversible mutations in gliding motility and virulence genes: A flexible and efficient phage defence mechanism in Flavobacterium psychrophilum.Environmental microbiology · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flavobacterium psychrophilum is an important fish pathogen in salmonid aquaculture worldwide. Due to increased antibiotic resistance, pathogen control using bacteriophages has been explored as a possible alternative treatment. However, the effective use of bacteriophages in pathogen control requires overcoming the selection for phage resistance in the bacterial populations. Here, we analyzed resistance mechanisms in F. psychrophilum after phage exposure using whole-genome sequencing of the ancestral phage-sensitive strain 950106-1/1 and six phage-resistant isolates. The phage-resistant strains had all obtained unique insertions and/or deletions and point mutations distributed among intergenic and genic regions. Mutations in genes related to cell surface properties, gliding motility, and biosynthesis of lipopolysaccharides and cell wall were found. The observed links between phage resistance and the genetic modifications were supported by direct measurements of bacteriophage adsorption rates, biofilm formation, and secretion of extracellular enzymes, which were all impaired in the resistant strains, probably due to superficial structural changes. The clustered regularly interspaced short palindromic repeat (CRISPR) region was unaffected in the resistant isolates and thus did not play a role as a resistance mechanism for F. psychrophilum under the current conditions. All together, the results suggest that resistance in F. psychrophilum was driven by spontaneous mutations, which were associated with a number of derived effects on the physiological properties of the pathogen, including reduced virulence under in vitro conditions. Consequently, phage-driven physiological changes associated with resistance may have implications for the impact of the pathogen in aquaculture, and these effects of phage resistance on host properties are therefore important for the ongoing exploration of phage-based control of F. psychrophilum.
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