ArticleBMC biotechnology2014
Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential.
Article in BMC biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed.
- Review
- Engineering Multi-Spheroid Bone Constructs Through the Fusion of Large MSC Spheroids.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- Click-crosslinked nanogels integrated into 3D stem cell spheroids enhance regenerative function for swallowing muscle repair.Biomaterials · 2026Article
- UNIQUE: ultrasound non-destructive in-situ quantitative evaluation of stem cell spheroid deformability during differentiation into specific lineages.Microsystems & nanoengineering · 2026Article
- Bone Organoids: A Novel Tool for Modeling and Managing Skeletal Disorders in Diabetes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- 3D Osteoimmune Stem Cell Spheroids with Osteoinduction and Immunomodulation Dual Functionality forACS biomaterials science & engineering · 2026Article
- Engineered 3D mesenchymal stem cell aggregates with multifunctional prowess for bone regeneration: Current status and future prospects.Journal of advanced research · 2026Review
- Comparison of 3D culture systems on mesenchymal stem cell longevity, regenerative potential, and secretome production.Regenerative medicine · 2025Article
- Organoid-based tissue engineering for advanced tissue repair and reconstruction.Materials today. Bio · 2025Review
- Developing a Bone-Mimicking Microenvironment: Surface Coating Method for Investigating Bone RemodelingACS biomaterials science & engineering · 2025Article
- Osteogenic differentiation of mesenchymal stem cells in cell-laden culture of self-assembling peptide hydrogels.OpenNano · 2025Article
- Effects of macrophages on the osteogenic differentiation of adipose tissue-derived stem cells in two-dimensional and three-dimensional cocultures.World journal of stem cells · 2025Article
- Spheroids from Epithelial and Mesenchymal Cell Phenotypes as Building Blocks in Bioprinting (Review).Sovremennye tekhnologii v meditsine · 2025Review
- A promising breakthrough in pancreatic cancer research: The potential of spheroids as 3D models.BioImpacts : BI · 2025Review
- Scaffold-free 3D culture systems for stem cell-based tissue regeneration.APL bioengineering · 2024Review
- Article
- Enhancing Bone Formation Through bFGF-Loaded Mesenchymal Stromal Cell Spheroids During Fracture Healing in Mice.Bioengineering (Basel, Switzerland) · 2024Article
- Synergistic Enhancement of Photodynamic Cancer Therapy with Mesenchymal Stem Cells and Theranostic Nanoparticles.ACS applied materials & interfaces · 2024Article
- Research progress on optimization ofFrontiers in bioengineering and biotechnology · 2024Review
- Mesenchymal stromal cells for bone trauma, defects, and disease: Considerations for manufacturing, clinical translation, and effective treatments.Bone reports · 2023Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMesenchymal stem cells (MSCs) are a favored cell source for regenerative medicine because of their multilinage potential. However, the conventional monolayer technique used to culture MSCs, inadequately overcomes their low differentiation capacity. Culture of MSCs in multicellular spheroids, more accurately mimics the in-vivo microenvironment; thus, resolving this problem. In this study, we assessed whether the osteoregenerative potential of MSC spheroids is greater than that of monolayer MSCs.
resultsMSC spheroids were generated from rat MSCs (rMSCs) using low-binding plates. Real-time reverse transcription-polymerase chain reaction and immunocytochemical analysis indicated that osteogenic properties were accelerated in MSC spheroids compared with monolayer rMSCs when treated with an osteoblast-inducer reagent for 7 days. Moreover, increased calcium deposition was visualized in MSC spheroids using Alizarin red staining. In a rat calvarial defect model, micro-computed tomography and histological assays showed that MSC spheroid-engrafted defects experienced enhanced bone regeneration.
conclusionsOur in-vitro and in-vivo results reveal that MSCs in the spheroid culture exhibit enhanced osteoregenerative efficiency compared with monolayer MSCs.
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