ArticlePloS one2014
Characterization of biosynthetic genes of ascamycin/dealanylascamycin featuring a 5'-O-sulfonamide moiety in Streptomyces sp. JCM9888.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Identification and distribution of a downregulatory signaling alkyloxazole inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Aminoacyl-tRNA-dependent enzymes in natural product biosynthesis: structure-function insights.The Journal of antibiotics · 2026Review
- Novel Antimicrobial Activities of Albofungin, Albonoursin, and Ribonucleosides Produced byMolecules (Basel, Switzerland) · 2025Article
- Identification of Genes Essential for Fluorination and Sulfamylation within the Nucleocidin Gene Clusters of Streptomyces calvus and Streptomyces virens.Chembiochem : a European journal of chemical biology · 2023Article
- Structural and Functional Insights into a Nonheme Iron- and α-Ketoglutarate-Dependent Halogenase That Catalyzes Chlorination of Nucleotide Substrates.Applied and environmental microbiology · 2022Article
- Biosynthesis of sulfonamide and sulfamate antibiotics in actinomycete.Journal of industrial microbiology & biotechnology · 2021Review
- Isolation of 5'-RSC advances · 2021Article
- Characterization of NucPNP and NucV involved in the early steps of nucleocidin biosynthesis inRSC advances · 2021Article
- Two 3'-Chemical science · 2019Article
- Progress and challenges in aminoacyl-tRNA synthetase-based therapeutics.The Journal of biological chemistry · 2019Review
- Aminoacyl sulfonamide assembly in SB-203208 biosynthesis.Nature communications · 2019Article
- Biosynthesis of 2'-Chloropentostatin and 2'-Amino-2'-Deoxyadenosine Highlights a Single Gene Cluster Responsible for Two Independent Pathways inApplied and environmental microbiology · 2017Article
- Heteroatom-Heteroatom Bond Formation in Natural Product Biosynthesis.Chemical reviews · 2017Review
- Nature's combinatorial biosynthesis and recently engineered production of nucleoside antibiotics in Streptomyces.World journal of microbiology & biotechnology · 2017Review
- Article
- Cameo appearances of aminoacyl-tRNA in natural product biosynthesis.Current opinion in chemical biology · 2016Review
- Natural and engineered biosynthesis of nucleoside antibiotics in Actinomycetes.Journal of industrial microbiology & biotechnology · 2016Review
- Purification and crystallographic analysis of a FAD-dependent halogenase from Streptomyces sp. JCM9888.Acta crystallographica. Section F, Structural biology communications · 2015Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ascamycin (ACM) and dealanylascamycin (DACM) are nucleoside antibiotics elaborated by Streptomyces sp. JCM9888. The later shows broad spectrum inhibition activity to various gram-positive and gram-negative bacteria, eukaryotic Trypanosoma and is also toxic to mice, while ascamycin is active against very limited microorganisms, such as Xanthomonas. Both compounds share an unusual 5'-O-sulfonamide moiety which is attached to an adenosine nucleoside. In this paper, we first report on the 30 kb gene cluster (23 genes, acmA to acmW) involved in the biosynthesis of these two antibiotics and a biosynthetic assembly line was proposed. Of them, six genes (AcmABGKIW) are hypothetical genes involved in 5'-O-sulfonamide formation. Two flavin adenine dinucleotide (FAD)-dependent chlorinase genes acmX and acmY were characterized which are significantly remote from acmA-W and postulated to be required for adenine C2-halogenation. Notably gene disruption of acmE resulted in a mutant which could only produce dealanylascamycin but was blocked in its ability to biosynthesize ascamycin, revealing its key role of conversion of dealanylascamycin to ascamycin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.