ArticlePloS one2014
Establishment of mammary gland model in vitro: culture and evaluation of a yak mammary epithelial cell line.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Caffeic Acid Counteracts LPS-Induced Inflammatory Damage in Yak Mammary Epithelial Cells Associated with NF-κB-Mediated Autophagy Regulation.Animals : an open access journal from MDPI · 2026Article
- Cellular characteristics and milk component productivity of primary bovine mammary cells for cell-cultured milk component production.Journal of animal science and technology · 2025Article
- Current status and challenges for cell-cultured milk technology: a systematic review.Journal of animal science and biotechnology · 2024Review
- The Role of Progesterone in Elf5 Activation and Milk Component Synthesis for Cell-Cultured Milk Production in MAC-T Cells.Animals : an open access journal from MDPI · 2024Article
- Dynamic Profile of the Yak Mammary Transcriptome during the Lactation Cycle.Animals : an open access journal from MDPI · 2023Article
- Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection.Cell communication and signaling : CCS · 2023Article
- Review
- House feeding system improves the estrus rate in yaks (Frontiers in microbiology · 2022Article
- Adaptation Mechanisms of Yak (Animals : an open access journal from MDPI · 2021Review
- Effect of Concentrate Supplementation on the Expression Profile of miRNA in the Ovaries of Yak during Non-Breeding Season.Animals : an open access journal from MDPI · 2020Article
- Development and validation of immortalized bovine mammary epithelial cell line as an in vitro model for the study of mammary gland functions.Cytotechnology · 2018Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to establish yak mammary epithelial cells (YMECs) for an in vitro model of yak mammary gland biology. The primary culture of YMECs was obtained from mammary gland tissues of lactating yak and then characterized using immunocytochemistry, RT-PCR, and western blot analysis. Whether foreign genes could be transfected into the YMECs were examined by transfecting the EGFP gene into the cells. Finally, the effect of Staphylococcus aureus infection on YMECs was determined. The established YMECs retained the mammary epithelial cell characteristics. A spontaneously immortalized yak mammary epithelial cell line was established and could be continuously subcultured for more than 60 passages without senescence. The EGFP gene was successfully transferred into the YMECs, and the transfected cells could be maintained for a long duration in the culture by continuous subculturing. The cells expressed more antimicrobial peptides upon S.aureus invasion. Therefore, the established cell line could be considered a model system to understand yak mammary gland biology.
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Registered trials
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