ArticlePloS one2014
Temporal and spatial stability of ammonia-oxidizing archaea and bacteria in aquarium biofilters.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- The Role of Ammonia-Oxidizing Archaea During Cycling and Animal Introduction in a Newly Commissioned Saltwater Aquarium.Animals : an open access journal from MDPI · 2025Article
- Microbial community succession of home aquarium biofilters associated with early establishment of comammoxISME communications · 2025Article
- ComammoxApplied and environmental microbiology · 2024Article
- Metagenomics and metabarcoding experimental choices and their impact on microbial community characterization in freshwater recirculating aquaculture systems.Environmental microbiome · 2023Article
- Ammonia-oxidizing archaea and complete ammonia-oxidizingWater research X · 2022Review
- Stochastic factors drive dynamics of ammonia-oxidizing archaeal and bacterial communities in aquaculture pond sediment.Frontiers in microbiology · 2022Article
- Article
- Microbial Community Succession and Nutrient Cycling Responses following Perturbations of Experimental Saltwater Aquaria.mSphere · 2019Article
- "Candidatus Nitrosotenuis aquarius," an Ammonia-Oxidizing Archaeon from a Freshwater Aquarium Biofilter.Applied and environmental microbiology · 2018Article
- Ammonia-oxidizing bacteria and archaea within biofilters of a commercial recirculating marine aquaculture system.AMB Express · 2018Article
- Cultivation and characterization of Candidatus Nitrosocosmicus exaquare, an ammonia-oxidizing archaeon from a municipal wastewater treatment system.The ISME journal · 2017Article
- Freshwater Recirculating Aquaculture System Operations Drive Biofilter Bacterial Community Shifts around a Stable Nitrifying Consortium of Ammonia-OxidizingFrontiers in microbiology · 2017Article
- Archaea in Natural and Impacted Brazilian Environments.Archaea (Vancouver, B.C.) · 2016Review
- Archaeal Community Changes Associated with Cultivation of Amazon Forest Soil with Oil Palm.Archaea (Vancouver, B.C.) · 2016Article
- Microbial biogeography of a university campus.Microbiome · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrifying biofilters are used in aquaria and aquaculture systems to prevent accumulation of ammonia by promoting rapid conversion to nitrate via nitrite. Ammonia-oxidizing archaea (AOA), as opposed to ammonia-oxidizing bacteria (AOB), were recently identified as the dominant ammonia oxidizers in most freshwater aquaria. This study investigated biofilms from fixed-bed aquarium biofilters to assess the temporal and spatial dynamics of AOA and AOB abundance and diversity. Over a period of four months, ammonia-oxidizing microorganisms from six freshwater and one marine aquarium were investigated at 4-5 time points. Nitrogen balances for three freshwater aquaria showed that active nitrification by aquarium biofilters accounted for ≥ 81-86% of total nitrogen conversion in the aquaria. Quantitative PCR (qPCR) for bacterial and thaumarchaeal ammonia monooxygenase (amoA) genes demonstrated that AOA were numerically dominant over AOB in all six freshwater aquaria tested, and contributed all detectable amoA genes in three aquarium biofilters. In the marine aquarium, however, AOB outnumbered AOA by three to five orders of magnitude based on amoA gene abundances. A comparison of AOA abundance in three carrier materials (fine sponge, rough sponge and sintered glass or ceramic rings) of two three-media freshwater biofilters revealed preferential growth of AOA on fine sponge. Denaturing gel gradient electrophoresis (DGGE) of thaumarchaeal 16S rRNA genes indicated that community composition within a given biofilter was stable across media types. In addition, DGGE of all aquarium biofilters revealed low AOA diversity, with few bands, which were stable over time. Nonmetric multidimensional scaling (NMDS) based on denaturing gradient gel electrophoresis (DGGE) fingerprints of thaumarchaeal 16S rRNA genes placed freshwater and marine aquaria communities in separate clusters. These results indicate that AOA are the dominant ammonia-oxidizing microorganisms in freshwater aquarium biofilters, and that AOA community composition within a given aquarium is stable over time and across biofilter support material types.
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