Evidence map›Paper›PMID 25478227›Full record

ArticleInternational journal of proteomics2014

Combined phosphoproteomics and bioinformatics strategy in deciphering drug resistant related pathways in triple negative breast cancer.

Xinyu Deng, Morris Kohanfars, Huan Ming Hsu, Puneet Souda, Joe Capri, Julian P Whitelegge, Helena R Chang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of proteomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Evidence of a synthetic lethality interaction between SETDB1 histone methyltransferase and CHD4 chromatin remodeling protein in a triple negative breast cancer cell line.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2023
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Xinyu DengGonda/UCLA Breast Cancer Research Laboratory and the Revlon/UCLA Breast Center, Department of Surgery, David Geffen School of Medicine, University of California at Los Angeles, 200 Med Plaza, Ste B265-1, Los Angeles, CA 90095-7028, USA.ORCID 0000-0002-3615-7371
Morris KohanfarsGonda/UCLA Breast Cancer Research Laboratory and the Revlon/UCLA Breast Center, Department of Surgery, David Geffen School of Medicine, University of California at Los Angeles, 200 Med Plaza, Ste B265-1, Los Angeles, CA 90095-7028, USA.ORCID 0000-0002-2150-9864
Huan Ming HsuGonda/UCLA Breast Cancer Research Laboratory and the Revlon/UCLA Breast Center, Department of Surgery, David Geffen School of Medicine, University of California at Los Angeles, 200 Med Plaza, Ste B265-1, Los Angeles, CA 90095-7028, USA.
Puneet SoudaThe Pasarow Mass Spectrometry Laboratory, NPI-Semel Institute, University of California at Los Angeles, Los Angeles, CA, USA.
Joe CapriThe Pasarow Mass Spectrometry Laboratory, NPI-Semel Institute, University of California at Los Angeles, Los Angeles, CA, USA.
Julian P WhiteleggeThe Pasarow Mass Spectrometry Laboratory, NPI-Semel Institute, University of California at Los Angeles, Los Angeles, CA, USA.
Helena R ChangGonda/UCLA Breast Cancer Research Laboratory and the Revlon/UCLA Breast Center, Department of Surgery, David Geffen School of Medicine, University of California at Los Angeles, 200 Med Plaza, Ste B265-1, Los Angeles, CA 90095-7028, USA.
University of California, Los Angeles · US

Funding

Transgenic & Knock-out MouseP30DK063491 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PETER J TONTONOZ · 2003 to 2026
$40.4M
NIDDK NIH HHS P30 DK063491
6 · The paper itself

Abstract

Because of the absence of a clear therapeutic target for triple negative breast cancer (TNBC), conventional chemotherapy is the only available systemic treatment option for these patients. Despite chemotherapy treatment, TNBC patients still have worse prognosis when compared with other breast cancer patients. The study is to investigate unique phosphorylated proteins expressed in chemoresistant TNBC cell lines. In the current study, twelve TNBC cell lines were subjected to drug sensitivity assays against chemotherapy drugs docetaxel, doxorubicin, gemcitabine, and cisplatin. Based on their half maximal inhibitory concentrations, four resistant and two sensitive cell lines were selected for further analysis. The phosphopeptides from these cells were enriched with TiO2 beads and fractionated using strong cation exchange. 1,645 phosphoprotein groups and 9,585 unique phosphopeptides were identified by a high throughput LC-MS/MS system LTQ-Orbitrap. The phosphopeptides were further filtered with Ascore system and 1,340 phosphoprotein groups, 2,760 unique phosphopeptides, and 4,549 unique phosphosites were identified. Our study suggested that differentially phosphorylated Cdk5, PML, AP-1, and HSF-1 might work together to promote vimentin induced epithelial to mesenchymal transition (EMT) in the drug resistant cells. EGFR and HGF were also shown to be involved in this process.

Identifiers

PMID25478227
PMCPMC4247952
OpenAlexW2144198445

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.