Evidence map›Paper›PMID 25477945›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2014

Impairments of mecA gene detection in bovine Staphylococcus spp.

Dayanne Araújo de Melo, Irene da Silva Coelho, Cássia Couto da Motta, Anna Carolina Coelho Marín Rojas, Felipe Carlos Dubenczuk, Shana de Mattos de Oliveira Coelho, Miliane Moreira Soares de Souza

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Frequently used therapeutic antimicrobials and their resistance patterns onInternational journal of veterinary science and medicine · 2022
    Article
  4. Accuracy of PCR universal primer for methicillin-resistant Staphylococcus and comparison of different phenotypic screening assays.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020
    Article
  5. Article
  6. Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dayanne Araújo de MeloDepartamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
Irene da Silva CoelhoDepartamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
Cássia Couto da MottaDepartamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
Anna Carolina Coelho Marín RojasDepartamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
Felipe Carlos DubenczukDepartamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
Shana de Mattos de Oliveira CoelhoDepartamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
Miliane Moreira Soares de SouzaDepartamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Staphylococcus aureus antimicrobial resistance, especially to beta-lactams, favors treatment failures and its persistence in herd environment. This work aimed to develop a more specific primer for mecA gene detection based on the comparison of the conserved regions from distinct host origins and also investigated the presence of homologue mecA(LGA251) in bovine strains. A total of 43 Staphylococcus spp. were included in this study, comprising 38 bovine S. aureus, two human and three equine coagulase-negative staphylococci (CNS). Phenotypical methicillin-resistance detection was performed through oxacillin agar-screening and cefoxitin disk-diffusion test. None isolate tested positive for mecA(LGA251) gene. For mecA gene PCR, new primers were designed based on the sequences of human S. aureus (HE681097) and bovine S. sciuri (AY820253) mecA. The new primers based on the S. aureus mecA sequence amplified fragments of human and equine CNS and the ones based on S. sciuri mecA sequence only yielded fragments for S. aureus bovine strains. Multiples alignments of mecA gene sequences from bovine, human and equine revealed punctual but significant differences in bovine strains that can lead to the mecA gene detection impairment. The observed divergences of mecA gene sequences are not a matter of animal or human origin, it is a specificity of bovine samples.

Indexed as

AnimalsBacterial ProteinsCattleDNA, BacterialDNA PrimersGenetic VariationHorsesHumansMethicillin ResistanceMicrobial Sensitivity TestsPenicillin-Binding ProteinsPolymerase Chain ReactionSequence AlignmentSequence Homology, Nucleic AcidStaphylococcal InfectionsStaphylococcus aureusBacterial ProteinsDNA, BacterialDNA PrimersmecA protein, Staphylococcus aureusPenicillin-Binding Proteinsantimicrobial resistancebovine mastitismecA geneStaphylococccus

Identifiers

PMID25477945
PMCPMC4204949

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.