ArticleFrontiers in plant science2014
Plant peroxisomes are degraded by starvation-induced and constitutive autophagy in tobacco BY-2 suspension-cultured cells.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- From Division to Death: Metabolomic Analysis ofPlants (Basel, Switzerland) · 2024Article
- Plant Heterotrophic Cultures: No Food, No Growth.Plants (Basel, Switzerland) · 2024Review
- ARP2/3 complex associates with peroxisomes to participate in pexophagy in plants.Nature plants · 2023Article
- Sulfadiazine and phosphinothricin selection systems optimised for the transformation of tobacco BY-2 cells.Plant cell reports · 2023Article
- Autophagic degradation of membrane-bound organelles in plants.Bioscience reports · 2023Review
- Product safety aspects of plant molecular farming.Frontiers in bioengineering and biotechnology · 2023Review
- Article
- Autophagy Is Involved in the Viability of Overexpressing ThioredoxinAntioxidants (Basel, Switzerland) · 2021Article
- Vacuolar processing enzyme translocates to the vacuole through the autophagy pathway to induce programmed cell death.Autophagy · 2021Article
- Multilevel Regulation of Peroxisomal Proteome by Post-Translational Modifications.International journal of molecular sciences · 2019Review
- Autophagic Machinery of Plant Peroxisomes.International journal of molecular sciences · 2019Review
- Selective Autophagy of Peroxisomes in Plants: From Housekeeping to Development and Stress Responses.Frontiers in plant science · 2019Review
- TOR-autophagy branch signaling via Imp1 dictates plant-microbe biotrophic interface longevity.PLoS genetics · 2018Article
- New advances in autophagy in plants: Regulation, selectivity and function.Seminars in cell & developmental biology · 2018Review
- Plant peroxisomes: recent discoveries in functional complexity, organelle homeostasis, and morphological dynamics.Current opinion in plant biology · 2016Review
- Pexophagy and peroxisomal protein turnover in plants.Biochimica et biophysica acta · 2016Review
- Morphological and quantitative changes in mitochondria, plastids, and peroxisomes during the log-to-stationary transition of the growth phase in cultured tobacco BY-2 cells.Plant signaling & behavior · 2016Article
- Over-expression of Trxo1 increases the viability of tobacco BY-2 cells under H2O2 treatment.Annals of botany · 2015Article
- Autophagy in plants and algae.Frontiers in plant science · 2014Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Very recently, autophagy has been recognized as an important degradation pathway for quality control of peroxisomes in Arabidopsis plants. To further characterize the role of autophagy in plant peroxisome degradation, we generated stable transgenic suspension-cultured cell lines of heterotrophic Nicotiana tabacum L. cv. Bright Yellow 2 expressing a peroxisome-targeted version of enhanced yellow fluorescent protein. Indeed, this cell line model system proved advantageous for detailed cytological analyses of autophagy stages and for quantification of cellular peroxisome pools under different culturing conditions and upon inhibitor applications. Complementary biochemical, cytological, and pharmacological analyses provided convincing evidence for peroxisome degradation by bulk autophagy during carbohydrate starvation. This degradation was slowed down by the inhibitor of autophagy, 3-methyladenine (3-MA), but the 3-MA effect ceased at advanced stages of starvation, indicating that another degradation mechanism for peroxisomes might have taken over. 3-MA also caused an increase particularly in peroxisomal proteins and cellular peroxisome numbers when applied under nutrient-rich conditions in the logarithmic growth phase, suggesting a high turnover rate for peroxisomes by basal autophagy under non-stress conditions. Together, our data demonstrate that a great fraction of the peroxisome pool is subject to extensive autophagy-mediated turnover under both nutrient starvation and optimal growth conditions. Our analyses of the cellular pool size of peroxisomes provide a new tool for quantitative investigations of the role of plant peroxisomes in reactive oxygen species metabolism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.