ReviewFrontiers in molecular neuroscience2014
Putting a finishing touch on GECIs.
Review in Frontiers in molecular neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
81 citing papers in PubMed.
- Precise calcium-to-spike inference using biophysical generative models.bioRxiv : the preprint server for biology · 2026Article
- Article
- The Mechanism of LTXInternational journal of molecular sciences · 2025Article
- Heterogeneous plasticity of amygdala interneurons in associative learning and extinction.Nature communications · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Generation of a HEK293 Cell Line with Stable Expression of Molecular Tools for Thermogenetic Activation.Bulletin of experimental biology and medicine · 2025Article
- Precise calcium-to-spike inference using biophysical generative models.Research square · 2025Article
- Spike rate inference from mouse spinal cord calcium imaging data.bioRxiv : the preprint server for biology · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Calcium Indicators with Fluorescence Lifetime-Based Signal Readout: A Structure-Function Study.International journal of molecular sciences · 2024Article
- Aberrant hippocampal CaeLife · 2024Article
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Aberrant hippocampal CabioRxiv : the preprint server for biology · 2024Article
- TIME-VARYINGData science in science · 2024Article
- Amplitude- and frequency-dependent activation of layer II/III neurons by intracortical microstimulation.iScience · 2023Article
- Astrocyte-specific CaBiochemistry and cell biology = Biochimie et biologie cellulaire · 2023Review
- A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation.Cell reports methods · 2023Article
- Development and characterization of novel jGCaMP8f calcium sensor variants with improved kinetics and fluorescence response range.Frontiers in cellular neuroscience · 2023Article
- Reduced excitatory neuron activity and interneuron-type-specific deficits in a mouse model of Alzheimer's disease.Communications biology · 2022Article
- Exploiting the molecular diversity of the synapse to investigate neuronal communication: A guide through the current toolkit.The European journal of neuroscience · 2022Review
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
More than a decade ago genetically encoded calcium indicators (GECIs) entered the stage as new promising tools to image calcium dynamics and neuronal activity in living tissues and designated cell types in vivo. From a variety of initial designs two have emerged as promising prototypes for further optimization: FRET (Förster Resonance Energy Transfer)-based sensors and single fluorophore sensors of the GCaMP family. Recent efforts in structural analysis, engineering and screening have broken important performance thresholds in the latest generation for both classes. While these improvements have made GECIs a powerful means to perform physiology in living animals, a number of other aspects of sensor function deserve attention. These aspects include indicator linearity, toxicity and slow response kinetics. Furthermore creating high performance sensors with optically more favorable emission in red or infrared wavelengths as well as new stably or conditionally GECI-expressing animal lines are on the wish list. When the remaining issues are solved, imaging of GECIs will finally have crossed the last milestone, evolving from an initial promise into a fully matured technology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.