Evidence map›Paper›PMID 25477555›Full record

ArticleChromatographia2014

Development of an Analytical Method for the Rapid Quantitation of Peptides Used in Microbicide Formulations.

Yufei Chen, Sidi Yang, Emmanuel A Ho

Open access · hybridAbstract read
In one paragraph

Article in Chromatographia, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Yufei ChenLaboratory for Drug Delivery and Biomaterials, Faculty of Pharmacy, University of Manitoba, Apotex Centre, 750 McDermot Ave, Room 329, Winnipeg, MB R3E 0T5 Canada.
Sidi YangLaboratory for Drug Delivery and Biomaterials, Faculty of Pharmacy, University of Manitoba, Apotex Centre, 750 McDermot Ave, Room 329, Winnipeg, MB R3E 0T5 Canada.
Emmanuel A HoLaboratory for Drug Delivery and Biomaterials, Faculty of Pharmacy, University of Manitoba, Apotex Centre, 750 McDermot Ave, Room 329, Winnipeg, MB R3E 0T5 Canada.
University of Manitoba · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recently, a growing number of macromolecules such as peptides and proteins have been formulated into various microbicide formulations for the prevention of sexually transmitted infections. However, a fast and reliable high-throughput method for quantitating peptide/protein in polymer-based microbicide formulations is still lacking. As a result, we developed and validated a reversed-phase high-performance liquid chromatography method for the quantitation of gp120 fragment and LL-37 simultaneously in various microbicide gel formulations. This method was capable of detecting a limit of linearity (regression coefficient of 0.999) for gp120 fragment and LL-37 within a range of 0.625-80 and 1.25-80 µg mL

Indexed as

Intravaginal gelProtein degradantReversed-phase HPLCSeparationTris-tricine SDS-PAGE

Identifiers

PMID25477555
PMCPMC4244548
OpenAlexW2092773979

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.