ArticleScience (New York, N.Y.)2014
Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein.
Article in Science (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 274 papers.
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Who cites it
274 citing papers in PubMed.
- Refinement of amino-acid conformation vs. difference density maps in time-resolved serial femtosecond crystallography data analysis.FEBS open bio · 2026Article
- Radiation damage in serial femtosecond crystallography studied in hemoglobin.Acta crystallographica. Section D, Structural biology · 2026Article
- Polyethylene Terephthalate Film-Based Sample Holder for Fixed-Target Serial Femtosecond Crystallography.Materials (Basel, Switzerland) · 2026Article
- Liquid-Microjet Photoelectron Spectroscopy of the Photoactive Yellow Protein Chromophore in Aqueous Solution.The journal of physical chemistry. A · 2026Article
- Time-resolved x-ray solution scattering from detergent solubilized visual rhodopsin.Biophysical journal · 2026Article
- Structural Mechanism of an Efficacy Photoswitch Targeting the βAngewandte Chemie (International ed. in English) · 2026Article
- Compact tape-driven sample delivery system for serial femtosecond crystallography.Journal of applied crystallography · 2026Article
- Automated microbatch-under-oil phase diagrams to rationalize serial crystallography sample preparation.IUCrJ · 2026Article
- Serial femtosecond crystallography data processing at the global science data hub center at KISTI.Scientific reports · 2026Article
- A Bright and Dynamic Future in Structural Biology.Advances in experimental medicine and biology · 2026Review
- Real-time capture of domain movements during copper amine oxidase catalysis by mix-and-inject serial crystallography.Nature communications · 2025Article
- Sensitive detection of structural dynamics using a statistical framework for comparative crystallography.Science advances · 2025Article
- Heavy-element damage seeding in proteins under XFEL illumination.Journal of synchrotron radiation · 2025Article
- Tracking Protein Motions using Serial Femtosecond Crystallography with X-Ray Free-Electron Laser.Current protocols · 2025Article
- Illuminating DNA repair in action: structural insights into a photocaged glycosylase complex.IUCrJ · 2025Article
- Structure Determination from Single-Molecule X-ray Scattering Images Using Stochastic Gradient Ascent.Journal of chemical theory and computation · 2025Article
- Preface to special topic: Celebrating the work and achievements of Keith Moffat.Structural dynamics (Melville, N.Y.) · 2025Article
- Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography.Nature methods · 2025Review
- Observation of early events in the photoactivation of Myxobacterial phytochrome using time-resolved serial femtosecond crystallography.Communications chemistry · 2025Article
- Advances in microfluidic mixers for time-resolved structural biology with X-rays.Biophysical reviews · 2025Review
214 more citing papers are in PubMed but not listed here.
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Abstract
Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.