ArticleNucleic acids research2015
Rho and RNase play a central role in FMN riboswitch regulation in Corynebacterium glutamicum.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Multi-omics analyses of evolvedSynthetic and systems biotechnology · 2026Article
- Rho-dependent transcription termination: mechanisms and roles in bacterial fitness and adaptation to environmental changes.RNA (New York, N.Y.) · 2025Review
- Immunization of mice and goats with Corynebacterium pseudotuberculosis-derived rPTS, rRBN and rCP40 recombinant proteins.World journal of microbiology & biotechnology · 2025Article
- TheRNA (New York, N.Y.) · 2024Article
- Riboswitch and small RNAs modulate btuB translation initiation in Escherichia coli and trigger distinct mRNA regulatory mechanisms.Nucleic acids research · 2024Article
- Direct and indirect control of Rho-dependent transcription termination by theRNA (New York, N.Y.) · 2024Article
- Engineering a Lactobacillus Lysine Riboswitch to Dynamically Control Metabolic Pathways for Lysine Production inMicroorganisms · 2024Article
- Internal transcription termination widely regulates differential expression of operon-organized genes including ribosomal protein and RNA polymerase genes in an archaeon.Nucleic acids research · 2023Article
- Regulation of Ribonuclease J Expression inJournal of bacteriology · 2022Article
- sRNA-mediated regulation of gal mRNA in E. coli: Involvement of transcript cleavage by RNase E together with Rho-dependent transcription termination.PLoS genetics · 2021Article
- Riboswitch Mechanisms: New Tricks for an Old Dog.Biochemistry. Biokhimiia · 2021Review
- Efficient production of long double-stranded RNAs applicable to agricultural pest control by Corynebacterium glutamicum equipped with coliphage T7-expression system.Applied microbiology and biotechnology · 2021Article
- Mastering the control of the Rho transcription factor for biotechnological applications.Applied microbiology and biotechnology · 2021Review
- Dual-level autoregulation of theRNA (New York, N.Y.) · 2020Article
- Article
- A multivariate prediction model for Rho-dependent termination of transcription.Nucleic acids research · 2018Article
- Maestro of regulation: Riboswitches orchestrate gene expression at the levels of translation, transcription and mRNA decay.RNA biology · 2018Review
- Translational control and Rho-dependent transcription termination are intimately linked in riboswitch regulation.Nucleic acids research · 2017Article
- Polynucleotide Phosphorylase, RNase E/G, and YbeY Are Involved in the Maturation of 4.5S RNA in Corynebacterium glutamicum.Journal of bacteriology · 2017Article
- Regulation of the Expression of De Novo Pyrimidine Biosynthesis Genes in Corynebacterium glutamicum.Journal of bacteriology · 2015Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Riboswitches are RNA elements that regulate gene expression in response to their ligand. Although these regulations are thought to be performed without any aid of other factors, recent studies suggested the participation of protein factors such as transcriptional termination factor Rho and RNase in some riboswitch regulations. However, to what extent these protein factors contribute to the regulation was unclear. Here, we studied the regulatory mechanism of the flavin mononucleotide (FMN) riboswitch of Corynebacterium glutamicum which controls the expression of downstream ribM gene. Our results showed that this riboswitch downregulates both ribM mRNA and RibM protein levels in FMN-rich cells. Analysis of mRNA stability and chromatin immunoprecipitation-real-time PCR analysis targeting RNA polymerase suggested the involvement of the mRNA degradation and premature transcriptional termination in this regulation, respectively. Simultaneous disruption of RNase E/G and Rho function completely abolished the regulation at the mRNA level. Also, the regulation at the protein level was largely diminished. However, some FMN-dependent regulation at the protein level remained, suggesting the presence of other minor regulatory mechanisms. Altogether, we demonstrated for the first time that two protein factors, Rho and RNase E/G, play a central role in the riboswitch-mediated gene expression control.
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