ArticleInfection and immunity1989
Effects of pyocyanine, a phenazine dye from Pseudomonas aeruginosa, on oxidative burst and bacterial killing in human neutrophils.
Article in Infection and immunity, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Genome-scale model of Pseudomonas aeruginosa metabolism unveils virulence and drug potentiation.Communications biology · 2023Article
- Pyocyanin induces NK92 cell apoptosis via mitochondrial damage and elevated intracellular CaInnate immunity · 2019Article
- Are reactive oxygen species always detrimental to pathogens?Antioxidants & redox signaling · 2014Review
- Unsuspected pyocyanin effect in yeast under anaerobiosis.MicrobiologyOpen · 2014Article
- Pyocyanin-enhanced neutrophil extracellular trap formation requires the NADPH oxidase.PloS one · 2013Article
- Hypoxia selectively inhibits respiratory burst activity and killing of Staphylococcus aureus in human neutrophils.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- One-pot three-component synthesis of quinoxaline and phenazine ring systems using Fischer carbene complexes.Beilstein journal of organic chemistry · 2010Article
- Total (bio)synthesis: strategies of nature and of chemists.Topics in current chemistry · 2010Review
- A strand-specific RNA-Seq analysis of the transcriptome of the typhoid bacillus Salmonella typhi.PLoS genetics · 2009Article
- Redox warfare between airway epithelial cells and Pseudomonas: dual oxidase versus pyocyanin.Immunologic research · 2009Article
- Liver sinusoidal endothelial cells and acute non-oxidative hepatic injury induced by Pseudomonas aeruginosa pyocyanin.International journal of experimental pathology · 2008Article
- Impairment of apoptotic cell engulfment by pyocyanin, a toxic metabolite of Pseudomonas aeruginosa.American journal of respiratory and critical care medicine · 2008Article
- A hampered chemoattractant-induced cytoskeletal rearrangement in granulocytes of patients with unexplained severe chronic and relapsing infections of the upper and lower airways. In vitro restoration by G-CSF exposure.Clinical and experimental immunology · 2002Article
- Modulation of neutrophil superoxide response and intracellular diacylglyceride levels by the bacterial pigment pyocyanin.Infection and immunity · 1997Article
- Role of oxidants in microbial pathophysiology.Clinical microbiology reviews · 1997Review
- Interaction of the Pseudomonas aeruginosa secretory products pyocyanin and pyochelin generates hydroxyl radical and causes synergistic damage to endothelial cells. Implications for Pseudomonas-associated tissue injury.The Journal of clinical investigation · 1992Article
- Leukotriene B4 omega-oxidation by human polymorphonuclear leukocytes is inhibited by pyocyanin, a phenazine derivative produced by Pseudomonas aeruginosa.Infection and immunity · 1992Article
- Production of leukotriene B4 and 5-hydroxyeicosatetraenoic acid by human neutrophils is inhibited by Pseudomonas aeruginosa phenazine derivatives.Infection and immunity · 1991Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of pyocyanine (phenazinium, 1-hydroxy-5-methyl-hydroxide, inner salt) on oxidative burst in human polymorphonuclear leukocytes were studied by several different approaches. In a cell- and enzyme-free system, pyocyanine oxidized NADPH. The reduced pyocyanine could be measured by its reaction with ferricytochrome c. It was shown by this assay that resting as well as phorbol myristate acetate- or zymosan-stimulated granulocytes reduced pyocyanine. The effect was independent of mitochondria, as cytoplasts were similarly active. Measurement of the hexose monophosphate shunt in intact granulocytes in the presence of pyocyanine indicated a concentration-dependent activation of the shunt without the generation of O2-, suggesting that pyocyanine oxidizes NADPH to NADP+ when it enters granulocytes. Intracellular NADPH in granulocytes was indeed lowered by almost 40% after incubation with pyocyanine. It is by this shuttling of reduction equivalents, leading to the partial depletion of NADPH, that pyocyanine affects the observed concentration-dependent partial inhibition of the phorbol myristate acetate- and zymosan-stimulated generation of O2-. A further consequence was that the intracellular killing of Staphylococcus aureus was also partially suppressed, particularly at higher loads of granulocytes with bacteria. Phagocytosis was not inhibited by pyocyanine concentrations as high as 500 microM. Pyocyanine did not affect the intracellular killing of Pseudomonas aeruginosa. The possible relevance of these findings to the course of mixed hospital infections in immunocompromised patients is discussed.
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