ArticleBMC genomics2014
Label-free proteomic analysis to confirm the predicted proteome of Corynebacterium pseudotuberculosis under nitrosative stress mediated by nitric oxide.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Proteomics of Toxigenic Corynebacteria.Proteomes · 2023Review
- Mycothiol maintains the homeostasis and signalling of nitric oxide in Streptomyces coelicolor A3(2) M145.BMC microbiology · 2023Article
- Multi-Omics ofProteomes · 2022Article
- Multi-omic data integration for the study of production, carcass, and meat quality traits in Nellore cattle.Frontiers in genetics · 2022Article
- Nitric Oxide Signaling for Actinorhodin Production in Streptomyces coelicolor A3(2) via the DevS/R Two-Component System.Applied and environmental microbiology · 2021Article
- A journey through thePeerJ · 2021Article
- Surface and Extracellular Proteome of the Emerging PathogenProteomes · 2018Article
- Mutation of the Surface Layer Protein SlpB Has Pleiotropic Effects in the ProbioticFrontiers in microbiology · 2018Article
- Transcriptome and Proteome of Fish-PathogenicFrontiers in microbiology · 2018Article
- Label-free quantitative proteomics of Corynebacterium pseudotuberculosis isolates reveals differences between Biovars ovis and equi strains.BMC genomics · 2017Article
- Whole-Genome Sequence ofGenome announcements · 2017Article
- A shift in the virulence potential of Corynebacterium pseudotuberculosis biovar ovis after passage in a murine host demonstrated through comparative proteomics.BMC microbiology · 2017Article
- Quantitative Proteomic Analysis Reveals Changes in the BenchmarkFrontiers in cellular and infection microbiology · 2017Article
- N-acetyl ornithine deacetylase is a moonlighting protein and is involved in the adaptation of Entamoeba histolytica to nitrosative stress.Scientific reports · 2016Article
- Changes in protein abundance are observed in bacterial isolates from a natural host.Frontiers in cellular and infection microbiology · 2015Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCorynebacterium pseudotuberculosis biovar ovis is a facultative intracellular pathogen, and the etiological agent of caseous lymphadenitis in small ruminants. During the infection process, the bacterium is subjected to several stress conditions, including nitrosative stress, which is caused by nitric oxide (NO). In silico analysis of the genome of C. pseudotuberculosis ovis 1002 predicted several genes that could influence the resistance of this pathogen to nitrosative stress. Here, we applied high-throughput proteomics using high definition mass spectrometry to characterize the functional genome of C. pseudotuberculosis ovis 1002 in the presence of NO-donor Diethylenetriamine/nitric oxide adduct (DETA/NO), with the aim of identifying proteins involved in nitrosative stress resistance.
resultsWe characterized 835 proteins, representing approximately 41% of the predicted proteome of C. pseudotuberculosis ovis 1002, following exposure to nitrosative stress. In total, 102 proteins were exclusive to the proteome of DETA/NO-induced cells, and a further 58 proteins were differentially regulated between the DETA/NO and control conditions. An interactomic analysis of the differential proteome of C. pseudotuberculosis in response to nitrosative stress was also performed. Our proteomic data set suggested the activation of both a general stress response and a specific nitrosative stress response, as well as changes in proteins involved in cellular metabolism, detoxification, transcriptional regulation, and DNA synthesis and repair.
conclusionsOur proteomic analysis validated previously-determined in silico data for C. pseudotuberculosis ovis 1002. In addition, proteomic screening performed in the presence of NO enabled the identification of a set of factors that can influence the resistance and survival of C. pseudotuberculosis during exposure to nitrosative stress.
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