ArticleScientific reports2014
Nuclemeter: a reaction-diffusion based method for quantifying nucleic acids undergoing enzymatic amplification.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- CRISPR-Programmable Nanoparticle Transport in Salt-Gated Porous Media for Visual DNA Detection.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2026Article
- A Monte Carlo simulation based on first-passage distributions for spatio-temporal detection on potentiometric sensor arrays.Analytical and bioanalytical chemistry · 2026Article
- Bridging the gap between development of point-of-care nucleic acid testing and patient care for sexually transmitted infections.Lab on a chip · 2022Review
- CFD simulations for paper-based DNA amplification reaction (LAMP) of Mycobacterium tuberculosis-point-of-care diagnostic perspective.Medical & biological engineering & computing · 2020Article
- Fully 3D printed integrated reactor array for point-of-care molecular diagnostics.Biosensors & bioelectronics · 2018Article
- Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests.Biosensors · 2018Review
- A Multifunctional Reactor with Dry-Stored Reagents for Enzymatic Amplification of Nucleic Acids.Analytical chemistry · 2018Article
- Is instrument-free molecular detection possible?Expert review of molecular diagnostics · 2017Article
- Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens.Nanoscale · 2017Article
- Miniaturized devices for point of care molecular detection of HIV.Lab on a chip · 2017Review
- Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection.ACS biomaterials science & engineering · 2016Article
- A high-efficiency superhydrophobic plasma separator.Lab on a chip · 2016Article
- Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette.PLoS neglected tropical diseases · 2015Article
- Integrated Microfluidic Nucleic Acid Isolation, Isothermal Amplification, and Amplicon Quantification.Microarrays (Basel, Switzerland) · 2015Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Real-time amplification and quantification of specific nucleic acid sequences plays a major role in medical and biotechnological applications. In the case of infectious diseases, such as HIV, quantification of the pathogen-load in patient specimens is critical to assess disease progression and effectiveness of drug therapy. Typically, nucleic acid quantification requires expensive instruments, such as real-time PCR machines, which are not appropriate for on-site use and for low-resource settings. This paper describes a simple, low-cost, reaction-diffusion based method for end-point quantification of target nucleic acids undergoing enzymatic amplification. The number of target molecules is inferred from the position of the reaction-diffusion front, analogous to reading temperature in a mercury thermometer. The method was tested for HIV viral load monitoring and performed on par with conventional benchtop methods. The proposed method is suitable for nucleic acid quantification at point of care, compatible with multiplexing and high-throughput processing, and can function instrument-free.
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Registered trials
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