Evidence map›Paper›PMID 25476898›Full record

ArticleCell death & disease2014

Enhancing mammary differentiation by overcoming lineage-specific epigenetic modification and signature gene expression of fibroblast-derived iPSCs.

Y Li, N Hong, A Zhang, W Chen, R-H Wang, X-L Xu, C-X Deng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell death & disease, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Y Li1] Genetics of Development and Disease Branch, NIDDK/NIH, 10/9N105, Bethesda, MD, USA [2] Xin-Qiao Hospital, The Third Military Medical University, Chongqing, China.
N HongGenetics of Development and Disease Branch, NIDDK/NIH, 10/9N105, Bethesda, MD, USA.
A ZhangGenetics of Development and Disease Branch, NIDDK/NIH, 10/9N105, Bethesda, MD, USA.
W ChenMicroarray Core Facility, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
R-H Wang1] Genetics of Development and Disease Branch, NIDDK/NIH, 10/9N105, Bethesda, MD, USA [2] Faculty of Health Sciences, University of Macau, Macau SAR, China.
X-L Xu1] Genetics of Development and Disease Branch, NIDDK/NIH, 10/9N105, Bethesda, MD, USA [2] Faculty of Health Sciences, University of Macau, Macau SAR, China.
C-X Deng1] Genetics of Development and Disease Branch, NIDDK/NIH, 10/9N105, Bethesda, MD, USA [2] Faculty of Health Sciences, University of Macau, Macau SAR, China.

Funding

Intramural NIH HHS
6 · The paper itself

Abstract

Recent studies have shown that induced pluripotent stem cells (iPSCs) retain a memory of their origin and exhibit biased differentiation potential. This finding reveals a severe limitation in the application of iPSCs to cell-based therapy because it means that certain cell types are not available for reprogramming for patients. Here we show that the iPSC differentiation process is accompanied by profound gene expression and epigenetic modifications that reflect cells' origins. Under typical conditions for mammary differentiation, iPSCs reprogrammed from tail-tip fibroblasts (TF-iPSCs) activated a fibroblast-specific signature that was not compatible with mammary differentiation. Strikingly, under optimized conditions, including coculture with iPSCs derived from the mammary epithelium or in the presence of pregnancy hormones, the fibroblast-specific signature of TF-iPSCs obtained during differentiation was erased and cells displayed a mammary-specific signature with a markedly enhanced ability for mammary differentiation. These findings provide new insights into the precise control of differentiation conditions that may have applications in personalized cell-based therapy.

Indexed as

Epigenesis, GeneticAnimalsCell DifferentiationCell LineageCells, CulturedCellular ReprogrammingCoculture TechniquesCpG IslandsDNA MethylationEpithelial CellsEstrogensFemaleFibroblastsGene Expression ProfilingInduced Pluripotent Stem CellsMammary Glands, AnimalEstrogensProgesterone

Identifiers

PMID25476898
PMCPMC4649828

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.