ArticleExperimental neurology2015
Respiratory function after selective respiratory motor neuron death from intrapleural CTB-saporin injections.
Article in Experimental neurology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Quantitative assessment of integrated phrenic nerve activity.Respiratory physiology & neurobiology · 2016Pooled it
- Impact of tongue exercise on hypoglossal axis survival, structure, and output in a rodent model of hypoglossal motor neuron degeneration.Journal of neurophysiology · 2026Article
- Spinal TNF-α receptor 1 is differentially required for phrenic long-term facilitation (pLTF) over the course of motor neuron death in adult rats.Frontiers in physiology · 2024Article
- Timeline of hypoglossal motor neuron death and intrinsic tongue muscle denervation in high-copy number SOD1Frontiers in neurology · 2024Article
- The Serotonergic System and Amyotrophic Lateral Sclerosis: A Review of Current Evidence.Cellular and molecular neurobiology · 2023Review
- AMPK-Nrf2 Signaling Pathway in Phrenic Motoneurons following Cervical Spinal Cord Injury.Antioxidants (Basel, Switzerland) · 2022Article
- Review
- Nonsteroidal anti-inflammatory drug (ketoprofen) delivery differentially impacts phrenic long-term facilitation in rats with motor neuron death induced by intrapleural CTB-SAP injections.Experimental neurology · 2022Article
- Phrenic motor neuron survival below cervical spinal cord hemisection.Experimental neurology · 2021Article
- Divergent receptor utilization is necessary for phrenic long-term facilitation over the course of motor neuron loss following CTB-SAP intrapleural injections.Journal of neurophysiology · 2021Article
- Clobetasol promotes neuromuscular plasticity in mice after motoneuronal loss via sonic hedgehog signaling, immunomodulation and metabolic rebalancing.Cell death & disease · 2021Article
- Tongue and hypoglossal morphology after intralingual cholera toxin B-saporin injection.Muscle & nerve · 2021Article
- Differential mechanisms are required for phrenic long-term facilitation over the course of motor neuron loss following CTB-SAP intrapleural injections.Experimental neurology · 2020Article
- 5-HT2A/B receptor expression in the phrenic motor nucleus in a rat model of ALS (SOD1Respiratory physiology & neurobiology · 2020Article
- Adenosine 2A receptor inhibition protects phrenic motor neurons from cell death induced by protein synthesis inhibition.Experimental neurology · 2020Article
- Mechanisms of compensatory plasticity for respiratory motor neuron death.Respiratory physiology & neurobiology · 2019Review
- Neuromuscular Plasticity in a Mouse Neurotoxic Model of Spinal Motoneuronal Loss.International journal of molecular sciences · 2019Article
- The Respiratory Phenotype of Rodent Models of Amyotrophic Lateral Sclerosis and Spinocerebellar Ataxia.Journal of neuroinflammation and neurodegenerative diseases · 2019Article
- Hypoglossal Motor Neuron Death Via Intralingual CTB-saporin (CTB-SAP) Injections Mimic Aspects of Amyotrophic Lateral Sclerosis (ALS) Related to Dysphagia.Neuroscience · 2018Article
- Phrenic long-term facilitation following intrapleural CTB-SAP-induced respiratory motor neuron death.Respiratory physiology & neurobiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Amyotrophic lateral sclerosis (ALS) causes progressive motor neuron degeneration, paralysis and death by ventilatory failure. In rodent ALS models: 1) breathing capacity is preserved until late in disease progression despite major respiratory motor neuron death, suggesting unknown forms of compensatory respiratory plasticity; and 2) spinal microglia become activated in association with motor neuron cell death. Here, we report a novel experimental model to study the impact of respiratory motor neuron death on compensatory responses without many complications attendant to spontaneous motor neuron disease. In specific, we used intrapleural injections of cholera toxin B fragment conjugated to saporin (CTB-SAP) to selectively kill motor neurons with access to the pleural space. Motor neuron survival, CD11b labeling (microglia), ventilatory capacity and phrenic motor output were assessed in rats 3-28days after intrapleural injections of: 1) CTB-SAP (25 and 50μg), or 2) unconjugated CTB and SAP (i.e. control; (CTB+SAP). CTB-SAP elicited dose-dependent phrenic and intercostal motor neuron death; 7days post-25μg CTB-SAP, motor neuron survival approximated that in end-stage ALS rats (phrenic: 36±7%; intercostal: 56±10% of controls; n=9; p<0.05). CTB-SAP caused minimal cell death in other brainstem or spinal cord regions. CTB-SAP: 1) increased CD11b fractional area in the phrenic motor nucleus, indicating microglial activation; 2) decreased breathing during maximal chemoreceptor stimulation; and 3) diminished phrenic motor output in anesthetized rats (7days post-25μg, CTB-SAP: 0.3±0.07V; CTB+SAP: 1.5±0.3; n=9; p<0.05). Intrapleural CTB-SAP represents a novel, inducible model of respiratory motor neuron death and provides an opportunity to study compensation for respiratory motor neuron loss.
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Registered trials
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