ArticleChest2015
A pilot study of the noninvasive assessment of the lung microbiota as a potential tool for the early diagnosis of ventilator-associated pneumonia.
Article in Chest, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 39 citations in OpenAlex.
- Microbiota Emergencies in the Diagnosis of Lung Diseases: A Meta-Analysis.Frontiers in cellular and infection microbiology · 2021Pooled it
- Emerging strategies for the noninvasive diagnosis of nosocomial pneumonia.Expert review of anti-infective therapy · 2019Pooled it
- Harnessing breath biomarkers for pneumonia diagnosis and prognosis.Expert review of respiratory medicine · 2026Review
- Research Priorities for Noninvasive Sampling of the Lower Respiratory Tract during Acute Respiratory Failure: An Official American Thoracic Society Workshop Report.Annals of the American Thoracic Society · 2025Article
- Review
- The lung microbiome: progress and promise.The Journal of clinical investigation · 2021Review
- Exhaled Breath Condensate-A Non-Invasive Approach for Diagnostic Methods in Asthma.Journal of clinical medicine · 2021Review
- Noninvasive sampling of the distal airspace via HME-filter fluid is not useful to detect SARS-CoV-2 in intubated patients.Critical care (London, England) · 2021Article
- Reconsidering ventilator-associated pneumonia from a new dimension of the lung microbiome.EBioMedicine · 2020Review
- Microbiomic Analysis on Low Abundant Respiratory Biomass Samples; Improved Recovery of Microbial DNA From Bronchoalveolar Lavage Fluid.Frontiers in microbiology · 2020Article
- Early treatment gains for antibiotic administration and within human host time series data.Mathematical medicine and biology : a journal of the IMA · 2018Article
- Novel Method for Noninvasive Sampling of the Distal Airspace in Acute Respiratory Distress Syndrome.American journal of respiratory and critical care medicine · 2018Article
- Ventilator Circuit Trash May Be a Research Treasure.American journal of respiratory and critical care medicine · 2018Article
- Lung Microbiota Is Related to Smoking Status and to Development of Acute Respiratory Distress Syndrome in Critically Ill Trauma Patients.American journal of respiratory and critical care medicine · 2018Article
- Variations of the lung microbiome and immune response in mechanically ventilated surgical patients.PloS one · 2018Article
- Microbiota in Exhaled Breath Condensate and the Lung.Applied and environmental microbiology · 2017Article
- Ventilator-Associated Pneumonia: The Role of Emerging Diagnostic Technologies.Seminars in respiratory and critical care medicine · 2017Review
- Host-Pathogen Interface: Progress in Understanding the Pathogenesis of Infection Due to Multidrug-Resistant Bacteria in the Intensive Care Unit.The Journal of infectious diseases · 2017Article
- The microbiome and critical illness.The Lancet. Respiratory medicine · 2016Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundVentilator-associated pneumonia (VAP) remains a common complication in critically ill surgical patients, and its diagnosis remains problematic. Exhaled breath contains aerosolized droplets that reflect the lung microbiota. We hypothesized that exhaled breath condensate fluid (EBCF) in hygroscopic condenser humidifier/heat and moisture exchanger (HCH/HME) filters would contain bacterial DNA that qualitatively and quantitatively correlate with pathogens isolated from quantitative BAL samples obtained for clinical suspicion of pneumonia.
methodsForty-eight adult patients who were mechanically ventilated and undergoing quantitative BAL (n = 51) for suspected pneumonia in the surgical ICU were enrolled. Per protocol, patients fulfilling VAP clinical criteria undergo quantitative BAL bacterial culture. Immediately prior to BAL, time-matched HCH/HME filters were collected for study of EBCF by real-time polymerase chain reaction. Additionally, convenience samples of serially collected filters in patients with BAL-diagnosed VAP were analyzed.
resultsForty-nine of 51 time-matched EBCF/BAL fluid samples were fully concordant (concordance > 95% by κ statistic) relative to identified pathogens and strongly correlated with clinical cultures. Regression analysis of quantitative bacterial DNA in paired samples revealed a statistically significant positive correlation (r = 0.85). In a convenience sample, qualitative and quantitative polymerase chain reaction analysis of serial HCH/HME samples for bacterial DNA demonstrated an increase in load that preceded the suspicion of pneumonia.
conclusionsBacterial DNA within EBCF demonstrates a high correlation with BAL fluid and clinical cultures. Bacterial DNA within EBCF increases prior to the suspicion of pneumonia. Further study of this novel approach may allow development of a noninvasive tool for the early diagnosis of VAP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.