ArticlePloS one2014
The predicted ABC transporter AbcEDCBA is required for type IV secretion system expression and lysosomal evasion by Brucella ovis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- Meta-Analysis and Advancement of Brucellosis Vaccinology.PloS one · 2016Pooled it
- Comparative Review of Brucellosis in Small Domestic Ruminants.Frontiers in veterinary science · 2022Review
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- When the Going Gets Rough: The Significance ofFrontiers in microbiology · 2021Review
- Transcriptome Landscape of Intracellular Brucella ovis Surviving in RAW264.7 Macrophage Immune System.Inflammation · 2020Article
- Pan-Proteomic Analysis and Elucidation of Protein Abundance among the Closely RelatedBiomolecules · 2020Article
- Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs.PloS one · 2020Article
- Investigation of host-pathogen interaction betweenCell & bioscience · 2017Article
- The ABC transporter YejABEF is required for resistance to antimicrobial peptides and the virulence of Brucella melitensis.Scientific reports · 2016Article
- Brucella canis is an intracellular pathogen that induces a lower proinflammatory response than smooth zoonotic counterparts.Infection and immunity · 2015Article
- Protection Provided by an Encapsulated Live Attenuated ΔabcBA Strain of Brucella ovis against Experimental Challenge in a Murine Model.Clinical and vaccine immunology : CVI · 2015Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Brucella ovis is a major cause of reproductive failure in rams and it is one of the few well-described Brucella species that is not zoonotic. Previous work showed that a B. ovis mutant lacking a species-specific ABC transporter (ΔabcBA) was attenuated in mice and was unable to survive in macrophages. The aim of this study was to evaluate the role of this ABC transporter during intracellular survival of B. ovis. In HeLa cells, B. ovis WT was able to survive and replicate at later time point (48 hpi), whereas an ΔabcBA mutant was attenuated at 24 hpi. The reduced survival of the ΔabcBA mutant was associated with a decreased ability to exclude the lysosomal marker LAMP1 from its vacuolar membrane, suggesting a failure to establish a replicative niche. The ΔabcBA mutant showed a reduced abundance of the Type IV secretion system (T4SS) proteins VirB8 and VirB11 in both rich and acid media, when compared to WT B. ovis. However, mRNA levels of virB1, virB8, hutC, and vjbR were similar in both strains. These results support the notion that the ABC transporter encoded by abcEDCBA or its transported substrate acts at a post-transcriptional level to promote the optimal expression of the B. ovis T4SS within infected host cells.
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Registered trials
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