ArticleInternational journal of molecular sciences2014
Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plant (Camellia sinensis (L.) O. Kuntze).
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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81 citing papers in PubMed, 195 citations in OpenAlex.
- Genome-wide identification and evolutionary analysis of the ERF-VII gene family in the tea plant (Camellia sinensis) and functional characterization of CsRAP2.2 in response to cold stress.Plant cell reports · 2026Article
- Lineage-specific diversification and tissue-specialized jasmonate defense roles of OPR genes in tea (Camellia sinensis).Molecular genetics and genomics : MGG · 2026Article
- Genome-wide association study and functional validation ofHorticulture research · 2026Article
- Reference Gene Stability inInternational journal of molecular sciences · 2026Article
- Effects of Light Quality on Anthocyanin Biosynthesis and Related Gene Expression inInternational journal of molecular sciences · 2025Article
- Stabilizing the Baseline: Reference Gene Evaluation in Three InvasiveInternational journal of molecular sciences · 2025Article
- Characterization of nitrate use efficiency in tea plant (Horticulture research · 2025Article
- Selection and validation of reference genes in alfalfa based on transcriptome sequence data.Scientific reports · 2025Article
- Transcriptomics and Plant Hormone Analysis Reveal the Mechanism of Branching Angle Formation in Tea Plants (International journal of molecular sciences · 2025Article
- Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis ofFood science & nutrition · 2025Article
- Transcriptomic analysis of hub genes regulating albinism in light- and temperature-sensitive albino tea cultivars 'Zhonghuang 1' and 'Zhonghuang 2'.Plant molecular biology · 2024Article
- Reference genes for qPCR expression in black tiger shrimp, Penaeus monodon.Molecular biology reports · 2024Article
- Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions.PloS one · 2024Article
- Selection and Validation of Reference Genes for Reverse-Transcription Quantitative PCR Analysis inInternational journal of molecular sciences · 2023Article
- Selection and validation of reliable reference genes for quantitative real-time PCR in Barnyard millet (Echinochloa spp.) under varied abiotic stress conditions.Scientific reports · 2023Article
- Transcriptome Analysis Reveals That Ascorbic Acid Treatment Enhances the Cold Tolerance of Tea Plants through Cell Wall Remodeling.International journal of molecular sciences · 2023Article
- CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants.International journal of molecular sciences · 2023Article
- Effect of the biosynthesis of the volatile compound phenylacetaldehyde on chloroplast modifications in tea (Horticulture research · 2023Article
- Topics and trends in fresh tea (Frontiers in plant science · 2023Review
- Uncovering the complex regulatory network of spring bud sprouting in tea plants: insights from metabolic, hormonal, and oxidative stress pathways.Frontiers in plant science · 2023Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reliable reference selection for the accurate quantification of gene expression under various experimental conditions is a crucial step in qRT-PCR normalization. To date, only a few housekeeping genes have been identified and used as reference genes in tea plant. The validity of those reference genes are not clear since their expression stabilities have not been rigorously examined. To identify more appropriate reference genes for qRT-PCR studies on tea plant, we examined the expression stability of 11 candidate reference genes from three different sources: the orthologs of Arabidopsis traditional reference genes and stably expressed genes identified from whole-genome GeneChip studies, together with three housekeeping gene commonly used in tea plant research. We evaluated the transcript levels of these genes in 94 experimental samples. The expression stabilities of these 11 genes were ranked using four different computation programs including geNorm, Normfinder, BestKeeper, and the comparative ∆CT method. Results showed that the three commonly used housekeeping genes of CsTUBULIN1, CsACINT1 and Cs18S rRNA1 together with CsUBQ1 were the most unstable genes in all sample ranking order. However, CsPTB1, CsEF1, CsSAND1, CsCLATHRIN1 and CsUBC1 were the top five appropriate reference genes for qRT-PCR analysis in complex experimental conditions.
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