ArticleLab on a chip2015
Integrated perfusion and separation systems for entrainment of insulin secretion from islets of Langerhans.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 64 citations in OpenAlex.
- Automated and online LC-MS/MS for multiplexed monitoring of hormone secretion from murine islets.Analytical and bioanalytical chemistry · 2026Article
- Label-Free Optical Sensor for Real-Time Monitoring of Insulin Secretion from Single Human Pancreatic Islets.Sensors (Basel, Switzerland) · 2026Article
- Unveiling islet heterogeneity using an automated microfluidic imaging system.Scientific reports · 2024Article
- Comparison between capillary electrophoresis and fluorescence anisotropy competitive immunoassay for glucagon.Electrophoresis · 2024Article
- A mechanism for slow rhythms in coordinated pancreatic islet activity.Biophysical journal · 2024Article
- A vascularized 3D model of the human pancreatic islet forBiofabrication · 2024Article
- Bone/cartilage organoid on-chip: Construction strategy and application.Bioactive materials · 2023Review
- Automated cellular stimulation with integrated pneumatic valves and fluidic capacitors.The Analyst · 2023Article
- Coordination of pancreatic islet rhythmic activity by delayed negative feedback.American journal of physiology. Endocrinology and metabolism · 2022Article
- Pancreatic islet organoids-on-a-chip: how far have we gone?Journal of nanobiotechnology · 2022Review
- Increasing insulin measurement throughput by fluorescence anisotropy imaging immunoassays.Analytica chimica acta · 2022Article
- Alternative Brain Slice-on-a-Chip for Organotypic Culture and Effective Fluorescence Injection Testing.International journal of molecular sciences · 2022Article
- Islet-on-a-chip for the study of pancreatic β-cell function.In vitro models · 2022Review
- The Foundation for Engineering a Pancreatic Islet Niche.Frontiers in endocrinology · 2022Review
- A microfluidic system for monitoring glucagon secretion from human pancreatic islets of Langerhans.Analytical methods : advancing methods and applications · 2021Article
- Building Biomimetic Potency Tests for Islet Transplantation.Diabetes · 2021Review
- A Microfluidic Hanging-Drop-Based Islet Perifusion System for Studying Glucose-Stimulated Insulin Secretion From Multiple Individual Pancreatic Islets.Frontiers in bioengineering and biotechnology · 2021Article
- Profiling Glucose-Stimulated and M3 Receptor-Activated Insulin Secretion Dynamics from Islets of Langerhans Using an Extended-Lifetime Fluorescence Dye.Analytical chemistry · 2020Article
- Rapid lipolytic oscillations in ex vivo adipose tissue explants revealed through microfluidic droplet sampling at high temporal resolution.Lab on a chip · 2020Article
- In Vitro Platform for Studying Human Insulin Release Dynamics of Single Pancreatic Islet Microtissues at High Resolution.Advanced biosystems · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A microfluidic system was developed to investigate the entrainment of insulin secretion from islets of Langerhans to oscillatory glucose levels. A gravity-driven perfusion system was integrated with a microfluidic system to deliver sinusoidal glucose waveforms to the islet chamber. Automated manipulation of the height of the perfusion syringes allowed precise control of the ratio of two perfusion solutions into a chamber containing 1-10 islets. Insulin levels in the perfusate were measured using an online competitive electrophoretic immunoassay with a sampling period of 10 s. The insulin immunoassay had a detection limit of 3 nM with RSDs of calibration points ranging from 2-8%. At 11 mM glucose, insulin secretion from single islets was oscillatory with a period ranging from 3-6 min. Application of a small amplitude sinusoidal wave of glucose with a period of 5 or 10 min, shifted the period of the insulin oscillations to this forcing period. Exposing groups of 6-10 islets to a sinusoidal glucose wave synchronized their behavior, producing a coherent pulsatile insulin response from the population. These results demonstrate the feasibility of the developed system for the study of oscillatory insulin secretion and can be easily modified for investigating the dynamic nature of other hormones released from different cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.