ArticleNature protocols2015
In vivo rapid gene delivery into postmitotic neocortical neurons using iontoporation.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- An unstable variant of GAP43 leads to neurodevelopmental deficiency.Scientific reports · 2024Article
- A low-cost device for cryoanesthesia of neonatal rodents.HardwareX · 2023Article
- Sparse postnatal labeling and quantification of superficial cortical cell synapses in the mouse neocortex.STAR protocols · 2022Article
- Microglia contribute to the postnatal development of cortical somatostatin-positive inhibitory cells and to whisker-evoked cortical activity.Cell reports · 2022Article
- Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development.Biology open · 2020Article
- A device for stereotaxic viral delivery into the brains of neonatal mice.BioTechniques · 2020Article
- Article
- Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation.Journal of visualized experiments : JoVE · 2017Article
- A cross-modal genetic framework for the development and plasticity of sensory pathways.Nature · 2016Article
- Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells.Biochemistry and biophysics reports · 2016Article
- Targeted in vivo genetic manipulation of the mouse or rat brain by in utero electroporation with a triple-electrode probe.Nature protocols · 2016Article
- Astroglial β-Arrestin1-mediated Nuclear Signaling Regulates the Expansion of Neural Precursor Cells in Adult Hippocampus.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol describes a method for directing the expression of genes of interest into postmitotic neocortical neurons in vivo. Microinjection of a DNA plasmid-amphiphilic molecule mix into the neocortex followed by delivery of an ad hoc electric pulse protocol during the first few days of life in mice allows rapid, focal and efficient expression of genes in postmitotic neurons. Compared with other gene delivery techniques such as in utero electroporation and viral infection, this method allows rapid (12 h), focal (50-200 μm), mosaic-like (50 to several hundred neurons) targeting of postmitotic neurons within existing circuits. This 'iontoporation' protocol, which can be completed within ∼20 min per mouse, allows straightforward assessment of genetic constructs in postmitotic cortical neurons and subsequent genetic, histological and physiological investigations of gene function.
Indexed as
Identifiers
25474030What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.