ArticleMolecular biology of the cell2015
A novel role for WAVE1 in controlling actin network growth rate and architecture.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- PQBP1-dependent alternative RNA splicing underlies high calorie diet-induced cognitive impairment.Molecular psychiatry · 2026Article
- Two ligands of Arp2/3 complex, yeast coronin and GMF, interact and synergize in pruning branched actin networks.The Journal of biological chemistry · 2025Article
- Evolutionary tuning of barbed end competition allows simultaneous construction of architecturally distinct actin structures.The Journal of cell biology · 2023Article
- ABI1-based expression signature predicts breast cancer metastasis and survival.Molecular oncology · 2022Article
- A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesis.Journal of cell science · 2022Article
- Single-molecule imaging of IQGAP1 regulating actin filament dynamics.Molecular biology of the cell · 2022Article
- Review
- WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge.Molecular biology of the cell · 2020Article
- GMF as an Actin Network Remodeling Factor.Trends in cell biology · 2018Review
- WH2 and proline-rich domains of WASP-family proteins collaborate to accelerate actin filament elongation.The EMBO journal · 2018Article
- 15α-methoxypuupehenol Induces Antitumor EffectsMolecular cancer therapeutics · 2017Article
- Cyclosporine A protects podocytes by regulating WAVE1 phosphorylation.Scientific reports · 2015Article
- Rho GTPase signalling in cell migration.Current opinion in cell biology · 2015Review
- Drosophila comes of age as a model system for understanding the function of cytoskeletal proteins in cells, tissues, and organisms.Cytoskeleton (Hoboken, N.J.) · 2015Review
- WASP family proteins: Molecular mechanisms and implications in human disease.European journal of cell biologyArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Branched actin filament networks in cells are assembled through the combined activities of Arp2/3 complex and different WASP/WAVE proteins. Here we used TIRF and electron microscopy to directly compare for the first time the assembly kinetics and architectures of actin filament networks produced by Arp2/3 complex and dimerized VCA regions of WAVE1, WAVE2, or N-WASP. WAVE1 produced strikingly different networks from WAVE2 or N-WASP, which comprised unexpectedly short filaments. Further analysis showed that the WAVE1-specific activity stemmed from an inhibitory effect on filament elongation both in the presence and absence of Arp2/3 complex, which was observed even at low stoichiometries of WAVE1 to actin monomers, precluding an effect from monomer sequestration. Using a series of VCA chimeras, we mapped the elongation inhibitory effects of WAVE1 to its WH2 ("V") domain. Further, mutating a single conserved lysine residue potently disrupted WAVE1's inhibitory effects. Taken together, our results show that WAVE1 has unique activities independent of Arp2/3 complex that can govern both the growth rates and architectures of actin filament networks. Such activities may underlie previously observed differences between the cellular functions of WAVE1 and WAVE2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.