Evidence map›Paper›PMID 25473086›Full record

ArticleStem cells translational medicine2015

In vitro cell motility as a potential mesenchymal stem cell marker for multipotency.

Alessandro Bertolo, Armin Gemperli, Marco Gruber, Benjamin Gantenbein, Martin Baur, Tobias Pötzel, Jivko Stoyanov

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

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  20. SIRT3 Enhances Mesenchymal Stem Cell Longevity and Differentiation.Oxidative medicine and cellular longevity · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Alessandro BertoloSwiss Paraplegic Research, Nottwil, Switzerland; Department of Health Sciences and Health Policy, University of Lucerne, Lucerne, Switzerland; Tissue and Organ Mechanobiology, Institute for Surgical Technology and Biomechanics, University of Bern, Bern, Switzerland; Cantonal Hospital of Lucerne, Lucerne, Switzerland; Swiss Paraplegic Centre, Nottwil, Switzerland.
Armin GemperliSwiss Paraplegic Research, Nottwil, Switzerland; Department of Health Sciences and Health Policy, University of Lucerne, Lucerne, Switzerland; Tissue and Organ Mechanobiology, Institute for Surgical Technology and Biomechanics, University of Bern, Bern, Switzerland; Cantonal Hospital of Lucerne, Lucerne, Switzerland; Swiss Paraplegic Centre, Nottwil, Switzerland.
Marco GruberSwiss Paraplegic Research, Nottwil, Switzerland; Department of Health Sciences and Health Policy, University of Lucerne, Lucerne, Switzerland; Tissue and Organ Mechanobiology, Institute for Surgical Technology and Biomechanics, University of Bern, Bern, Switzerland; Cantonal Hospital of Lucerne, Lucerne, Switzerland; Swiss Paraplegic Centre, Nottwil, Switzerland.
Benjamin GantenbeinSwiss Paraplegic Research, Nottwil, Switzerland; Department of Health Sciences and Health Policy, University of Lucerne, Lucerne, Switzerland; Tissue and Organ Mechanobiology, Institute for Surgical Technology and Biomechanics, University of Bern, Bern, Switzerland; Cantonal Hospital of Lucerne, Lucerne, Switzerland; Swiss Paraplegic Centre, Nottwil, Switzerland.
Martin BaurSwiss Paraplegic Research, Nottwil, Switzerland; Department of Health Sciences and Health Policy, University of Lucerne, Lucerne, Switzerland; Tissue and Organ Mechanobiology, Institute for Surgical Technology and Biomechanics, University of Bern, Bern, Switzerland; Cantonal Hospital of Lucerne, Lucerne, Switzerland; Swiss Paraplegic Centre, Nottwil, Switzerland.
Tobias PötzelSwiss Paraplegic Research, Nottwil, Switzerland; Department of Health Sciences and Health Policy, University of Lucerne, Lucerne, Switzerland; Tissue and Organ Mechanobiology, Institute for Surgical Technology and Biomechanics, University of Bern, Bern, Switzerland; Cantonal Hospital of Lucerne, Lucerne, Switzerland; Swiss Paraplegic Centre, Nottwil, Switzerland.
Jivko StoyanovSwiss Paraplegic Research, Nottwil, Switzerland; Department of Health Sciences and Health Policy, University of Lucerne, Lucerne, Switzerland; Tissue and Organ Mechanobiology, Institute for Surgical Technology and Biomechanics, University of Bern, Bern, Switzerland; Cantonal Hospital of Lucerne, Lucerne, Switzerland; Swiss Paraplegic Centre, Nottwil, Switzerland jivko.stoyanov@paraplegie.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stem cells (MSCs) are expected to have a fundamental role in future cell-based therapies because of their high proliferative ability, multilineage potential, and immunomodulatory properties. Autologous transplantations have the "elephant in the room" problem of wide donor variability, reflected by variability in MSC quality and characteristics, leading to uncertain outcomes in the use of these cells. We propose life imaging as a tool to characterize populations of human MSCs. Bone marrow MSCs from various donors and in vitro passages were evaluated for their in vitro motility, and the distances were correlated to the adipogenic, chondrogenic, and osteogenic differentiation potentials and the levels of senescence and cell size. Using life-image measuring of track lengths of 70 cells per population for a period of 24 hours, we observed that slow-moving cells had the higher proportion of senescent cells compared with fast ones. Larger cells moved less than smaller ones, and spindle-shaped cells had an average speed. Both fast cells and slow cells were characterized by a low differentiation potential, and average-moving cells were more effective in undergoing all three lineage differentiations. Furthermore, heterogeneity in single cell motility within a population correlated with the average-moving cells, and fast- and slow-moving cells tended toward homogeneity (i.e., a monotonous moving pattern). In conclusion, in vitro cell motility might be a useful tool to quickly characterize and distinguish the MSC population's differentiation potential before additional use.

Indexed as

BiomarkersCell DifferentiationCell MovementCells, CulturedCellular SenescenceFlow CytometryHumansIn Vitro TechniquesMesenchymal Stem CellsMultipotent Stem CellsTime-Lapse ImagingBiomarkersDifferentiation potentialHuman mesenchymal stem cellsIn vitro cell motilityStem cell transplantation

Identifiers

PMID25473086
PMCPMC4275012

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.