ArticleNature2014
Deconstructing transcriptional heterogeneity in pluripotent stem cells.
Article in Nature, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 217 papers.
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Who cites it
217 citing papers in PubMed.
- Single-cell marker gene clustering: A unified deep learning framework for marker gene-based clustering of single-cell RNA-sequencing data.Quantitative biology (Beijing, China) · 2026Article
- Ligand-based directed differentiation to produce granulosa-like cells expressing steroidogenic enzyme genes.Stem cell research · 2026Article
- Transcriptional Bursting in Pluripotent Stem Cells.Biology · 2026Review
- The RNA binding protein LIN28A mediates chromatin dynamics during neuronal differentiation.Cell death and differentiation · 2026Article
- Slow RNAPII elongation enhances naive pluripotency rewiring while maintaining high replication fork speed.Science advances · 2026Article
- iceDP: identifying inter-chromatin engagement via density peaks clustering algorithm.Briefings in bioinformatics · 2026Article
- Progress and applications of single-cell RNA sequencing and spatial transcriptome technology in acute kidney injury research.Molecular therapy. Nucleic acids · 2025Review
- CeiTEA: Adaptive Hierarchy of Single Cells with Topological Entropy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Topology entropy: Enhancing graph partitioning for TAD identification and single-cell clustering.Computational and structural biotechnology journal · 2025Article
- GEMLI: Gene Expression Memory-Based Lineage Inference from Single-Cell RNA-Sequencing Datasets.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Unravelling lipid heterogeneity: Advances in single-cell lipidomics in cellular metabolism and disease.BBA advances · 2025Review
- EpiCHAOS: a metric to quantify epigenomic heterogeneity in single-cell data.Genome biology · 2024Article
- Lung Immune Cell Niches and the Discovery of New Cell Subtypes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Single-cell RNA sequencing in diabetic kidney disease: a literature review.Renal failure · 2024Review
- IDclust: Iterative clustering for unsupervised identification of cell types with single cell transcriptomics and epigenomics.NAR genomics and bioinformatics · 2024Article
- Redox heterogeneity in mouse embryonic stem cells individualizes cell fate decisions.Developmental cell · 2024Article
- scLENS: data-driven signal detection for unbiased scRNA-seq data analysis.Nature communications · 2024Article
- Gene-expression memory-based prediction of cell lineages from scRNA-seq datasets.Nature communications · 2024Article
- Combining Global-Constrained Concept Factorization and a Regularized Gaussian Graphical Model for Clustering Single-Cell RNA-seq Data.Interdisciplinary sciences, computational life sciences · 2024Article
- Unraveling the signaling mechanism behind astrocytoma and possible therapeutics strategies: A comprehensive review.Cancer reports (Hoboken, N.J.) · 2023Review
157 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors.
Funding
Abstract
Pluripotent stem cells (PSCs) are capable of dynamic interconversion between distinct substates; however, the regulatory circuits specifying these states and enabling transitions between them are not well understood. Here we set out to characterize transcriptional heterogeneity in mouse PSCs by single-cell expression profiling under different chemical and genetic perturbations. Signalling factors and developmental regulators show highly variable expression, with expression states for some variable genes heritable through multiple cell divisions. Expression variability and population heterogeneity can be influenced by perturbation of signalling pathways and chromatin regulators. Notably, either removal of mature microRNAs or pharmacological blockage of signalling pathways drives PSCs into a low-noise ground state characterized by a reconfigured pluripotency network, enhanced self-renewal and a distinct chromatin state, an effect mediated by opposing microRNA families acting on the Myc/Lin28/let-7 axis. These data provide insight into the nature of transcriptional heterogeneity in PSCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.